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5 Cards in this Set

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What does the mediator do? What special property does it have?
It is a large protein complex does not make direct contact with DNA, but makes protein-protein contacts, such as a bridge between activator protein and the transcriptional machinery. It can transmit instructions that increase or decrease the rate of transcription to the transcriptional machinery.
What does the chromatin remodeling complexe do?
What do they need to function?
It will modify the path of the DNA around the histone octamer and cause nucleosome sliding.
It requires ATP
What are the three types of the chromatin remodeling complexes and what do they do?
SWI/SNF: switch/sniff, it will modify the path of the DNA around the histone octamer and cause nucleosome sliding.

ISWI (imitation of switch): displace the nucleosomes without changing its structure.

SWR: take a H2A-H2B dimer and modify H2A into H2A.Z-H2B. These dimmers will create a signal to the transcriptional machinery, telling it this is the place that needs to be transcribed.
Once the Transcriptional machinery is at the basal promoter, it still cannot transcribe without trouble, as there are other nucleosome along the way. What additional complex is there and how does it work?
FACT: : release H2A-H2B dimmers from the nucleosomes, and the nucleosome disassemble. Which creates a hole ahead of the pol II, so it can transcribe. Once done transcribing, FACT helps to reassemble the nucleosome behind the RNA pol II.
What does the histone acetyltransferase do, and how does this help with the overall transcription along with histone kinase? (hint, what do they create?)
It acetylate the histone tail on a specific amino acid, and kinase phosphorylate histone tail. Together this creates a histone code, which can be recognized by general transcriptional factors such as TFIID. This tells the transcriptional machinery that this is the region that needs to be transcribed.