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9 Cards in this Set

  • Front
  • Back

trp operon

Repressible negative regulatory protein.


(Anabolism= synthesis of tryptophan)


Tryptophan binds to the repressor, forming a complex that binds to the operator to inhibit transcription.

Lac operon

Consists of a promoter, operator and three structural genes (Z, Y, and A).


(Catabolism= breakdown of lactose)


Lactose binds to the receptor, forming a complex that releases from the operator, inducing gene expression.



Lactose

One transcription regulation pathway of


bacteria. When the lactose gene is present and switched on, anabolism (build up) occurs.

Lactose

Inducible negative regulatory protein.


When lactose concentration is low, the lac


repressor binds to the operator, preventing RNA polymerase from transcribing the


structural gene to break down lactose.

Lac repressor

Inducible negative regulatory protein.


When lactose concentration is low, the lac


repressor binds to the operator, preventing RNA polymerase from transcribing the


structural gene to break down lactose.

Allolactose

When lactose concentration is high, allolactose is produced and binds to an allosteric site of lac repressor, changing its conformation so that it cannot bind to the operator. This allows RNA polymerase to transcribe and continue breaking down lactose.

Arabinose

Another transcription regulation pathway of


bacteria. When the arabinose gene is present and switched on, catabolism (break down)


occurs.

Galactoside Permease

Channel in E. Coli that allows bacteria to take in lots of lactose into the cell, where it can be cleaved by galactosidase to produce glucose and galactose. This induces the expression of these genes.

Negative & Positive Gene Regulation

Positive gene regulation= regulatory proteins main function is to enhance gene expression.


-catabolism?




Negative gene regulation= regulatory proteins main function is to inhibit gene expression.


-can be inducible or repressible