Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
9 Cards in this Set
- Front
- Back
|
Koch's molecular postulate #1 |
P1) gene or product is only found in strains of bacteria that cause disease |
|
|
Koch's molecular postulate #2 |
P2) gene should be isolated by cloning |
|
|
Koch's molecular postulate #3 |
P3) positive isolated gene to non-virulent strain = makes virulent and inactivation of the gene in wt = eliminates virulence |
|
|
Koch's molecular Postulate #4 |
P4) gene is expressed by bacterium during infection in humans or animals |
|
|
Transposon Mutagenesis |
Introducing mutations that insert a piece of DNA into any given gene and k/out entire gene -labelled insertion site with identifiable sequence and an Ar marker |
|
|
Transposing Q^R |
In all different situations = k/out of a different gene - can look at which gene affects invasive ness - can also k/out gene and provide a plasmid with active gene and see if complement action occurs |
|
|
Transposing limits: |
-if introduce Tn terminator into 1st gene = k/out 1st gene and entire downstream sequence -insertion cannot k/o genes essential for growth on serum used for selection |
|
|
PCR advantages |
- quicker and cheaper than cultivation based tests -increased sensitivity (single bacterium is sufficient) -can detect organism that's dead and cannot be cultivated |
|
|
DNA microarrays |
Looks at a bunch of genes all at once -looks at which genes are turned on in different environments/situations |
