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15 Cards in this Set

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Be able to list and diagram the parts of a microscope (16).
-ocular lens -ocular eyepiece -diopter ring -revolving nosepiece -eyepiece sleeve -objectives -mechanical stage with travel knobs -coarse and fine adjustment knobs -condensor -rheostat -arm -base -stage
What are the 6 working regions of a microscope?
-objectives (red, yellow/green, blue, white)
-specimen stage
-substage condenser and light source
-focus control
How can you control intensity and quality of light reaching the objective?
-rheostat (on-off switch)
-iris diaphragm (open has lower contrast, better resolve)
When is the greatest intesity of light on the specimen in terms of the condenser?
Where should it be positioned for stained and wet mounts?
-when it's at its highest position
*stained smears: 102mm below slide
*wet mounts: lowest level
Who made the first microscope?
-Anton Van Leeuwenhoek
What is the microscope we will mostly be using in this lab?
-the compound brightfield microscope
What are the three lens' used in todays compound microscope?
-objective lens
-ocular lens
Why does the 100X need oil?
-It can't take in enough light to be useful
-oil has similar refractive index so light isn't scattered as much and results in greater resolution
What are the markings found on each objective?
-E=type of objective
-100=objective magnification
-1.25 oil=numerical aperture
-160/0.17= mechanical tube length/cover glass thickness correction
How do you find the resolution for a microscope?
wavelength light/(NA obj lens + NA con lens)
What is the calculation for finding NA?
NA= n sin(theta)
n= refractive index of medium between specimen and lens
sin(theta)= sin function of half the total angle of the cone of light trapped by the lens
What are the two fixed faxtors when calculating the resolution of a lens?
-wavelength of visible light
-angle of incident light rays trapped by objective can not be greater than 60 degrees (sin < 0.866)
What does parfocal mean?
-When switching from low to higher power, image should be nearly focused
*use only fine focus knobs!
What should you check before putting the scope back in its cabinet?
-4x objective over hole
-clean all lenses
-using kimwipe, clean any oil or debris off stage
-fold cord and put under stage on base
-center slide older
-cover microscope
Be able to describe how to observe a sample in the microscope.
pg's 9 and 10 in Appendix A