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9 Cards in this Set
- Front
- Back
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Transformation requries
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Need competent recipient cell
Need recognition of self |
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Uptake of naked donor DNA from environment
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Transformation
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Transfer of DNA from donor to recipient cell via a bacteriophage
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Transduction
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-Lytic pathway where small fragments of host cell DNA can be accidently packaged into new phages
-Any bacterial chrom. gene has equal chance of being transduced -Low freq transduction |
Generalized transduction
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-Lysogenic pathway where prophage integrates-->de-integrates when want to reproduce & picks up piece of bacterial chrom and transfers into new host
-high frequency transduction |
Specialized transuction
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Conjugation btw F+ & F-
Plasmid recirculizes & replicates independently High freq. plasmid transfer |
Standard conjugation
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-F plasmid integrated into host
-btw Hfr donor & F- -length of contact determines amt. of chrom. transferred -high freq. transfer of chrom. genes |
Hfr
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virus that infects & integrates their DNA into bacterial cell chrom.
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Lysogeny (bacteriophage)
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Found in pathogens, not in non-virulent bacteria
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Pathogenicity islands
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