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12 Cards in this Set
- Front
- Back
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What reactions are enzymes involved in?
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breakdown, synthesis, and chemical transfers
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What is the Michaelis-Menton constant?
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Km=Vmax/2
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What is the Michaelis-Menton equation?
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Vo=Vmax(S)/(Km+(S))
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What is the Michaelis-Menton linear equation? (Lineweaver-Burke)
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1/Vo=Km/(Vmax(S))+1/Vmax
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What is NP-40 used for in Enzyme Kinetics?
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enzyme extraction buffer (lyse?)
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How do you find Vo when you have more than one sample?
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Plot the concentrations vs. time
Vo=slope |
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What is the relationship between Vo and enzyme amount?
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direct
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What is the relationship between Vo and substrate amount?
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direct until saturation
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What is the equation for transformation efficiency?
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((# of colonies)X(dilution factor))/ug DNA
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What does araC do on the GFP plasmid?
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It makes it so the gene is only transcribed in presence of arabinose.
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What does bla do on the GFP plasmid?
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It protects the transformed cells from death by ampicillin.
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What 6 things are needed to do PCR?
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DNA template
nucleotides Taq Magnesium Primers Salt Buffer |
