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24 Cards in this Set

  • Front
  • Back
best media used to screen GBS, group B strep
LIM broth
differentiate among clinically relevant alpha hemolytic strep
S. pnuemoniae is bile soluble optochin susceptible
Viridans strep is bile insoluble optochin resistant
differentiate group D strep from Enterococcus
both postive bile eschilin test but Enterococcus is only one of two that grows in 6.5% NaCl broth
biochemical test for presumptive or definative id of beta hemolyticc gram positve cocci in chains
bile esculin, PYR, bacitracin, hippurate, CAMP, VP
group B strep most commonly associated w what disease
meningitis in neonates and elderly
biochemical differentiation among clinically relevant beta hemolytic strep
S. pyogenes positive PYR
S. agalactiae positive Hipp and CAMP
cause of exudative pharygitis
S. pyogenes
characteristic of Enterococcus from group D strep
Enterococci relatively resistant to antibiotics, ie penicillin
Enterococci grows in 6.5% NaCl broth
organism considered universally susceptible to penicillin
S. pyogenes
beta hemolytic gram-positive cocci isolated from neonate
S. agalactiae
most appropiate culture media to select and differentiate Salmonella
bismuth sulfite
sputum gram stain demonstrating numerous neutrophils and small, pleomorphic gram-negative bacilli suggests
Haemophilus, most likely H. influenzae coming from sputum, respiratory infection
least bochemically reactive member of Enterobacteriaceae
Shiggella spp
Enterobacteriaceae that produces H2S
Salmonella spp
colorless growth on MAC, yellow colonies on HE agar, acid/acid TSI with no gas or H2S, possible pathogen
gram-negative non-lactose fermentor, sucrose fermentor indicates Yersinia spp
TSI reactions alkaline slant, acid butt, no H2S, no gas produced not what?
1.
2.
1. Salmonella spp because produce H2S
2. Yersinia spp or E. coli because produce acid/acid TSI
acid slant/acid butt on TSI no H2S no gas, possible enterics
1.
2.
how differentiate?
1. E. coli (lactose fermentor)
2. Yersinia spp
organism cause severe cholera-like symptoms through production of enterotoxins
E. coli
group D shigella that produce capsule
procedure to remove capsule for antisera agglutination?
S. sonnei
boiling organism
VP positive, nonmotile, gram-negative bacilli
Klebsiella
differentiation of P. aeruginosa among other Pseudomonas spp
P. aeruginosa grows at 42*C
oxidase and catalase positive nonmotile gram-negative bacilli which optimally grows at 42*C
Campylobacter jejuni
species isolated on chocolate agar from patient w epiglottitis possible pathogens
1.
2.
3.
how differentiate?
1. H. influenzae
2. H. parainfluenzae requires V but not X factor
3. H. haemolyticus is hemolytic
differentiation between Acinebacter spp and Moraxella spp
Acinetobacter is oxidase negative
Moraxella spp is oxidase positive