Mltt 203 Microbiology Text

Front 1

catalase-postive, gram-positve cocci

Back 1

Staphylococcus and Micrococcus

Front 2

most virulent staphylococci encountered

Back 2

Staph aureus

Front 3

UTI coagulase-negative staphylococci. how differentiated from other CNS?

Back 3

Stapy saprophyticus
novobiocin susceptible

Front 4

general gram-stain morphology of staphylococci

Back 4

gram positive cocci in clusters

Front 5

identification of Staph aureus. exception?

Back 5

catalase-positive, gram-positive cocci in clusters forming white to yellow, cream, opaque colonies on BAP that is slide and tube coagulase positive in 4 hours.
-exception: coagulase-positive staph isolated from dog bite, suspect S. intermedius

Front 6

similarities between Streptococcus and Enterococcus

Back 6

gram-positive cocci seen in chains and catalase-negative

Front 7

hemolysis pattern of Strep pyogenes on BAP. What factors cause this pattern?

Back 7

beta hemolytic caused by stroptolysin O and S

Front 8

common poststreptococcal disease resulting from Strep pyogenes

Back 8

acute glomerulonephritis and rheumatic fever

Front 9

primary cause of bacterial pneumonia. gram stain morphology of bacteria

Back 9

Strep pneumoniae: gram-positive, lancet-shaped cocci occuring singly, in pairs, or short chains

Front 10

define catalse. associated with what gram-positive cocci?

Back 10

enzyme that catalyzes H2O2 into O2 and H2O.
catalase-positive GPC: Staph; catalase-negative GPC: Strep & Enterococci

Front 11

Most catalase-negative gram-positive cocci grow on what media with exception to what catalase-negative GPC?

Back 11

BAP and chocolate except Abiotrophia and Granulicatella AKA "nutritionally defficient" Strep

Front 12

differentiate between Staph and Micrococcus

Back 12

Staph is susceptible to furazolidone and Miccrococcus is resistant

Front 13

What is Group A and Group B strep? How differentiate?

Back 13

Group A: S.pyogenes; Group B: S. agalactiae
S. pyogenes is bacitracin sensitive and S. agalactiae is bacitracin resistant

Front 14

special processing of specimens suspected of Bacillus spp.

Back 14

heat or alcohol shock to increase chances of laboratory isolation of spore forming Bacillus spp

Front 15

should Bacillus spp be grown on MAC or CNA?

Back 15

neither; MAC selects for gram-negative bacteria and CNA can inhibit nalidixic acid susceptible spp

Front 16

media for isolation and id of B. anthracis

Back 16

PLET and bicarbonate agar

Front 17

biochemical test that differentiates B. anthracis from B. cereus

Back 17

B. antracis is nonmotile and B. cereus is motile

Front 18

morphological differences from B. anthracis and B. cereus

Back 18

B. antracis is nonhemolytic and B. cerues is beta-hemolytic

Front 19

catalase-positive, gram-positive rods that don't form spores, survives within phagocytes

Back 19

Listeria monocytogenes

Front 20

catalase-positive, gram-positive rods that dont form spores, produces extremely potent cytotoxic exotoxin

Back 20

Corynebacterium diphtheriae

Front 21

cold enrichment is a procedure using a nutrient broth at 4*C for weeks to months to aid in the isolation of?

Back 21

Listeria monocytogenes from placental tissue

Front 22

general characteristics of Enterobacteriaceae. exception?

Back 22

gram-negative, glucose fermentor, oxidase-negative, reduce nitrates to nitrites and catalse positive straight rods with rounded ends
-exception: Shigella dysenteriae is catalase-negative

Front 23

members of Enterobacteriaceae that are considered overt pathogens

Back 23

Salmonella typhi: typhoid fever; Shigella spp.: dysentery; and Y. pestis: "black" plague

Front 24

gram-negative coccobacilli resembling a closed safety pin

Back 24

Y. pestis

Front 25

proper media for cultivation of E. coli 0157:H7

Back 25

MacConkey-Sorbitol agar

Front 26

special conditions for cultivation of Y. enterocolitica

Back 26

48 hours on CIN (cefsulodin-irgasan-novobiocin)

Front 27

"bulls-eye" colonies on CIN after 48 hours incubation indicates what organisms
1.
2.
how differentiate?

Back 27

1. Y. enterocolitica (oxidase-negative)
2. Aeromonas spp (oxidase-positive)

Front 28

second and third most commonly encountered gram-negative bacilli in clinical specimens. how differentiated?

Back 28

Acinetobacter spp and stenotrophomonas maltophilia (greenish pigment on BAP)

Front 29

how Acinetobacter spp and Stenotrophomonas maltophilia differentiated from Enterobacteriaceae?

Back 29

Enterobacteriacea ferment glucose

Front 30

Vibrio choleae characteristic in growth requirement

Back 30

does not require salt for growth

Front 31

most commonly encountered organism in clinical specimens that are a part of animal normal flora, transmitted by traumatic inoculation through human skin

Back 31

Pasteurella multocida

Front 32

gram-negative bacilli/coccobacilli susceptible to penicillin

Back 32

Pasteurella spp

Front 33

define HACEK and name organisms

Back 33

group of organisms that cause slowly progressive bacterial endocarditis
-Haemophilus aphrophilus
-Actinobacillus actinomycetemcomitans
-Cardiobacterium hominis
-Eikenello corrodens
-Kingella spp

Front 34

gram-negative bacilli requiring "X" and "V" factor for in vitro growth

Back 34

Haemophilus spp

Front 35

environmental conditions for Haemophilus to grow on chocolate agar

Back 35

5%-10% CO2

Front 36

gram-negative rods appearing curved or "seagull-winged" shaped

Back 36

Camplyobacter spp

Front 37

falcultative, intracellular parasite associated with domesticated livestock

Back 37

Brucella

Front 38

gram-negative diplococci that do not elongate whe exposed to subinhibitory concentrations of penicillin. oxidase reaction?

Back 38

Neiserria gonorrhoeae and N. meningitidis, Moraxella catarrholis
-oxidase-positive

Front 39

large, nonpigmented or grey colony cultured on chocolate agar can me moved intact over the surface of the agar

Back 39

presumptively Moraxella catarrhalis

Front 40

biochemical test that differentiates coccoid Neisseria spp

Back 40

N. gonorrhoeae negative maltose, N. meningitidis postive maltose

Front 41

culture media conditions of gram-negative diplococci. exception?

Back 41

BAP and chocolate in CO2 enriched, humid environment
-exception: N. gonorrhoeae more fastidious, plate on MTM or NYC medium

Front 42

carbohydrate profile of Moraxella catarrhalis

Back 42

inert; glucose, maltose, lactose negative

Front 43

gram-stain procedure alteration for anaerobic bacteriology

Back 43

safranin counterstain is left on for 3-5 min

Front 44

culture media for C. difficile

Back 44

CCFA cycloserine cefoxitin fructose agar