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30 Cards in this Set

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Eukaryote (define & example)

An organism with a true nucleus, DNA, and organelles enclosed in a membrane. Some have a cell wall. Examples: animals, plants, fungi, algae.

YOU-karyote

Prokaryote

A single celled organism without a membrane bound nucleus and with minimal organelles. Example: bacteria.

Three most used taxonomic ranks, and their structure (largest to smallest.)

Family: 1st letter capitalized, all have -aceae ending. (Streptococcaceae)



Genus: 1st letter capitalized, and underlined OR italicized. (Streptococcus)



Species: no caps, underline OR italicize. (pneumoniae)

FGS

Cocci

Bacteria with a spherical shape.

Bacilli

Bacteria that are rod shaped.

Coccobacilli

A shape between cocci and bacilli, easily mistaken for cocci.

Arrangements of cocci

5

Vibrio

Curved rod shaped bacteria (like macaroni.)

Spirilla & spirochetes

Spiral shaped bacteria. The first is more wavy, and the latter more corkscrew in shape.

Pleomorphism (define & example)

Bacteria with a variety of shapes and sizes, for example Haemophilus influenziae.

The process of bacterial reproduction

Reproduction is asexual, with every generation doubling the amount of bacteria. Growth is exponenrial.

Flagellum (definition & function)

A whip-like appendage protruding from bacteria. They aid movement by rotational movement (not whipping back and forth,) with clockwise rotation moving them forwards and counterclockwise rotation flipping outward and changing direction. Can have multiple flagellum.



They have antigens, so we produce antibodies against them. This also allows for antigenic typing.



Generally only rods have flagella.

Chemotaxis

Movement of bacteria due to a chemical stimulus. Away from toxic environments and towards nutrient sources.

Monotrichous

Having only one flagella.

Lophotrichous

Having multiple flagella in the same area.

Amphitrichous

Having one flagella on each end.

Peritrichous

Having multiple flagella in multiple locations.

Brownian Movement

False, non-directional motion. Caused when cells are bombarded by water molecules. Truly motile bacteria have flagella.

Pili vs Fimbriae

Both primarily seen in Gram negative bacteria. They help to attach to a host cell. Without attachment, bacteria can wreak havoc but not cause infection.




They are shorter than flagella, and fimbriae are shorter than pili.




Pili may aid in genetic diversity. F-pili is a hollow tube which can join and transmit genetic information between bacteria.

Capsule

Not all bacteria have a capsule. It is a slick, slimy covering outside of the bacteria cell wall. It's composition is mainly polysaccharide, and it is huge compared to the bacteria.




It is mainly for protection - WBC's slide off. Thus they can continue living and causing infection. Capsules do not stain.

Cell Wall

Mainly contributes to structural integrity of bacteria, so they don't lyse and are able to survive in salinity, (about 0.85% on average.)




Main structural components are N-acetylglucosamine and N-acetylmuramic acid, alternating to make up peptidoglycan.

Gram + Cell Wall

Made up of 60-100% peptidoglycan. It is thick, but simple.




Teichoic acid (T.A.) extends through cell wall and helps regulate cell wall synthesis.

Gram = cell wall

Only about 5-10% peptidoglycan. 20-30% is phospholipid, and 40-50% is made up of protein.




It is more complex, but thinner, than gram + cell walls.

Protein in Cell Walls

It is the most important part of the cell wall, and is highly likely to cause the body to produce antibodies.




It stimulates fever, lowering blood presuure, and can cause D.I.C.

Cytoplasmic Membrane

Made up of phospholipids and proteins. Regulates passage of substances into and out of the cell.

Porins

A protein that cross the membrane and act as a pore through which molecules can diffuse.

Ribosomes

Serves as the site of mRNA translation and protein synthesis.

Plasmids

Extrachromasomal DNA that can give bacteria extra abilites.

Endospores

A survival tool - can survive in this form for long periods of time when environment is unfavorable.

Gram Stain

  • Crystal Violet for 10 seconds, then rinse. This is the primary stain.
  • Gram's Iodine for 20 seconds, then rinse. This sets the dye.
  • Decolorize with Acetone/Alcohol, immediately rinse. Gram + will retain violet.
  • Counter stain with Safranin for 10 seconds, then rinse. This stains gram = red/pink.



Gram pos is purple.


Gram neg is red (bank account in the red is negative!)