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34 Cards in this Set
- Front
- Back
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in phenol red broth this yellow color
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acid fermentation
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in phenol red broth this red color means
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no fermentation
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in phenol red broth this pink color means
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break down of peptone
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in phenol red pH < 6.8 is
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yellow
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in phenol red pH > 7.4 is
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pink/magenta
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in phenol red pH 6.8 - 7.4
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red
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This test detects organisms capable of mixed acid fermentation
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Methyl Red (MR)
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what is the idicatior/dye of MR test
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methyl red
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red color in MR is what type of result
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positive
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yellow color in MR is what type of result
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negative
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orange color in MR is what type of result
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negative/inconclusive
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this test is for organisms capable of fermenting glucose but convert their acid products to acetonin and 2-3 butanediol
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VP
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what does a red color mean in the VP test
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positive result and 2-3 butanediol was fermented and acetoin was made
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what does a copper color or no color change mean in the VP test
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negative result and 2-3 butanediol was not fermented and acetoin was not made
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what selective media isolates Gram + bacteria
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MSA
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what is the pH indicator in MSA
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phenol red
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what promotes staphylococci growth but inhibits gram - growth in MSA
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NaCl
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what does red growth mean in MSA
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can't ferment mannitol
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what selective media isolates Gram -
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EMB
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what is EMB used to test for
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coliforms
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what inhibits gram + bacteria in EMB
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eosin and methylene blue
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what is the indicator in the Citrate test
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bromthymol blue
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what does a green color mean in the citrate test
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negative, citrate not utilized
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what does a blue color mean in the citrate test
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positive test citrate utilized
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this is the colored molecule in simple stains
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chromagen
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this gives chromagen its color
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chromatophore
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this is the charged portion of chromagen that allow it to act as a dye
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auxochrome
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this is a test to see if mycolic acid is present in the cell wall
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acid fast stain
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what is the primary stain in the acid fast stain
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carbolfucshin
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what is the counterstain in acid fast stain
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methylene blue or brilliant green
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this test takes advantage of the fact that mucoid polysaccharide/polypeptide is resistant to most staining
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capsule stain
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what is the stain of capsule staining
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congo red or nigrosin
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what is the primary stain of endospore stain
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malachite green
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what is the counterstain of endospore stain
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safranin
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