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15 Cards in this Set
- Front
- Back
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Name aceptic tecniques.
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1)bonami soap on slides
2)disinfectent liquid on tables before and after use 3)flame loop and needle before and after use |
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Explain streak plate for isolation
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purpose: isolate colines and certin type of bacteria
get 1 inoculum then streak, flame, streak, flame, streak, flame |
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Transfer instraments
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transfer loop
transfer needle |
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Smear from broth culture
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flame, cool, get loop full of bacteria and wipe on slide in a circulaton motion to make a dime size sample
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Simple stain
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clean w/bonami, then heat dry to take off oil
add smear heat fix crystal violet 1min, rince, blot dry |
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How is the simple stain able to hold on?
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the crystal violet is atracted to the LPS found in Gram - bacteria. The LPS creates a negative net charge out side the cell.
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What are the main cell shapes
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Cocci
Rod Spiral |
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Gram stain
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Prepare slide
Crystal violet - primary stain for G(+) Lugols iodine - mordant Ethal alcohol - decolorizer Safranin - counter stain |
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Compare and contrast G(-) and G(+)
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Gram (-)
thin layer of peptioglycan = thin cell wall stained red/pink Outer membrane consists of LPS, lipids, and protiens. Lipoprotiens help bind the peptidoglycan ot the uter membrane LPS creates a net negative charge around the cell Periplasmic space contains hydrolytic and degrading enzymes along with sugars and proteins Gram positive bacteria are stained purple No cell membrane Teichoic and Lipidteichoic acids in the eptidoglycan layer to create a net negative charge around the cell Major characteristic in both Plasma membrane/ inner membrane - consists of a phospholipid bilayer and contains proteins |
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Why expose plate to air
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air has bacteria
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History of Gram Stain
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1884 Cristian Gram
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Charaeristic of bacteria
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Light transmission - opaque or translucent
Surface - smooth, wrinkled, concentric, contoured Elevation - raised or convex Shape - circular, punctiform irregular, rhizoid Margin - straight, undulate, irregular, filamentous, curled Odor - earthy, fruity, ammonia Pigmented - color Gram stain |
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Name the shapes of a colony
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filamentous
irregular round rhyzoid curled |
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Name the edges of a colony
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entire
filamentous irregular (undulate, lobate) |
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Name the elevations of a colony
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raised
flat convet umbonate growth int medium |
