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32 Cards in this Set

  • Front
  • Back
Definition of a Microbe?
Organism that usually cannot be seen with the naked eye.
≤0.1mm = 100 µm
Cellular and noncellular
Role of Microbes in environment
High diversity, numbers and biomass
photosynthesis
major recyclers
biogeochemical cycles
produce/consume greenhouse gases
Impact of microbes on humans
Disease in plants/animals
Normal flora
Agriculture
Food and beverage
Bioremediation
Fuel source
Industry (enzymes, antibiotics, chemicals)
How old is the planet?
4.6 billion years
How long ago did the first prokaryotic cell originate?
3.8 bya
how long ago did the first eukaryotic cell originate?
2 bya
What microbial group was the first oxygenic photosynthesizer to evolve?
Oxygenic photosynthetic bacteria:
3 bya, cyanobacteria

Oxygenic photosynthetic eukaryotices:
1.5 bya, algae
Where do bacteria exist?
Nearly everywhere;
Water necessary
How many total bacteria are thought to inhabit the planet?
10^30
van Leeuwenhoek
Significantly improved quality of microscope lenses
First to observe very small microbes, including bacteria
Pasteur
Father of microbiology
Disproved spontaneous generation
Described scientific basis of fermentation
Developed pasteurization
developed several vaccines, including rabies
Robert Koch
Demonstrated certain diseases caused by microbes
Developed criteria used to prove a disease is caused by a specific microbe (Koch's postulates)
Edward Jenner
1789: first to develop vaccine (small pox)
Carl Woese
Described taxonomy based on evolutionary relationships
Molecules are documents of evolutionary history
Taxonomy based on genotype
Discovered archaea
Koch's Postulates
To show cause of a particular disease
1) pathogen present in every case
2) pathogen isolated from diseased host and grown in pure culture
3) pathogen inoculated into healthy host causes same disease
4) organism must be re-isolated and shown to be same organism
Problems with Koch's postulates
Lack of animal model
Several pathogens cannot be grown in culture
Carl Woese's important discoveries
Three domains of life: bacteria, archaea, and eukarya
2/3 domains have prokaryotic cell types
archaea more evolutionary related to eukarya than to bacteria
Discovered archaea
How did Carl Woese's work change how life is categorized?
Regrouped into three domains, based on evolutionary relatedness (genotype)

(previously based on phenotype)
How to write genus, species name
Underlined or italicized
Genus capitalized, species lowercase
Similarities and differences between bacteria and archaea
Cannot be distinguished with microscope
Similarities:
Lack nucleus and complex cell structure of eukarya
Small size
simple shapes
high metabolic diversity
asexual reproduction

Differences:
Bacteria have peptidoglycan in cell walls, archaea have none
Domain Bacteria
General characteristics
Prokaryotes (no nucleus)
Small cells; simple shapes
Unicellular
Asexual reproduction
Cell wall = peptidoglycan
Generates energy using light, organic chemicals or inorganic chemicals (depends on species)
Can grow in wide variety of conditions
Some are pathogens
Domain Archaea
General characteristics
Prokaryotes
If cell walls, no peptidoglycan
Many similar properties as Bacteria:
Lack nucleus & complex cell structure of Eukarya
Small size
Simple shapes
High metabolic diversity
Asexual reproduction
Distinct from Bacteria: more similar to Eukarya biochemically & genetically
All unicellular
NO KNOWN PATHOGENS
Domain Eukarya
General characteristics
Most unicellular
Larger than prokaryotes, more varied cell shapes
Some are pathogens
Eukarya: Protista
Protozoa: first animal
lack cell walls
sexual &/or asexual reproduction
some independent, some parasites
algae=photosynthetic
Eukarya: Fungi
Eukaryotic cell type
not plants! no photosynthesis
absorb organics for food
cell walls of chitin
sexual and asexual reproduction
some are pathogens
Unicellular yeasts
multicellular molds
mushrooms make hyphae
Eukarya: Plantae
NO pathogens
Photosynthetic
Cell walls of cellulose
Multicellular or Unicellular
Eukarya: Animalia
Multicellular
Lack cell walls
Non-photosynthetic
Ingest other cells for food
Mostly macroscopic
Which domain do viruses belong?
None
Binomial nomenclature
Developed by carolus linnaeus (1735)
Latin names; taxonomy
rRNA
Ribosomal RNA
All organisms have rRNA in cells
Ribosomes perform same essential functions in all cells--make proteins
Genes encoding rRNA contain:
conserved region (allows gene to be identified easily)
variable region (determines evolutionary relationships)
taxonomy
science of classification
phylogeny
evolutionary relationships between organisms.