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21 Cards in this Set
- Front
- Back
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Blood Agar
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Enrichment and Differential
Sheep Red Blood Cells hemolysins nonhemoletic-Enterococcus faecalis alpha hemolysis - incomplete dissolution of red cells-Streptococcus mitis beta hemolysis = complete dissolution - Streptococcus Group E |
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Mannitol Salt
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Selective Differential
Staphylococcus = + S. epidermidis = negative |
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MacConkey Agar
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red colonies = lactose fermenters -- E. aerogenes, K. pneumoniae
RED COLONIES + PRECIPITATION = E. Coli Colorless/no lactose -- Salmonella, Shigella, Proteus |
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EMB
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Lactose: E. Coli - blue-black colonies with green metallic sheen
E. aerogenes, K. pneumoniae both pink colonies Nonlactose-colorless --- Salmonella, Shigella, Proteus |
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Triple Sugar Iron (TSI) Slant
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K/NC = negative
K/A = glucose positive A/A = lactose and/or sucrose positive gas = cracks in agar black precipitate = H2S positive |
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Lactose Fermentation
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yellow = positive
AG = E. Coli, Klebsiella & Enterobacter Negative = Salmonella, Shigella, and Proteus |
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Indole Production
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red = positive test - E. Coli
no color - neg. test - Klebsiella and enterobacter |
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Methyl Red
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E. Coli, Shigella, Proteus, and Salmonella = +
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Voges-Proskauer (VP)
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Enterobacter Aerogenes = +
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Citrate Test
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growth = blue slant + for E. aerogenes, K. Pneumoniae, Salmonella, Proteus, Psuedomonas aeruginosa.
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Hydrogen Sulfide Production
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Black precipitate = +
= Salmonella, and Shigella |
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Motility
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E. Coli, Enterobacter, salmonella, proteus, psuedamonas aeruginosa, alcaligenes faecalis
Klebsiella & Shigella are negative |
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Urease Test
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Deep Pink = positive -- Proteus, K. pneumonia are only positive ones
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Casein
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cleared section around growth meaning hydrolysis of peptide bonds has occurred = only psuedomonas aeruginosa
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Nitrate Reaction
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Red w/o Zinc = Positive
No color after Zinc added = positive Only negative for this test is Alcaligenes Faecalis |
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Catalase Reaction
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bubbles = positive test
only negative for shigella and streptococcus lactis |
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Oxidase Reaction
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positive = pink maroon-black within 30 minutes
+ only for Psuedamonas aeruginosa and alacaligenes faecalis |
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Starch Hydrolysis
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Added Gram's Iodine and Clear Zone around = positive test
None are positive except Bacillus cereus |
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Litmus Milk
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lactose ferment = pink
white = reduction of litmus; excess H+ production, alkaline = blue. |
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Gelatin Hydrolysis
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liquid = positive test. Positive = B. cereus and P. aeruginosa and proteus mirabilis
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Glucose Hydrolysis
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Yellow = fermentation
Positive = E. Coli, E. aerogenes, K. pneumoniae, Proteus, and Salmonella |
