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40 Cards in this Set

  • Front
  • Back

Obligate anaerobes have enzymes such as superoxide dismutase to protect them from the damaging effects of oxygen

false

Nitrogen fixation is a process that occurs in all bacteria

false

thermoduric mesophiles are often responsible for spoilage of improperly canned foods

true

Serial dilution can be used in combination with pour plates as a method for isolating pure cultures

true

the only effective way to store bacterial cultures for short periods of time is to arrest their metabolism by freezing

false

Quorum sensing is involved in the development and propagation of a biofilm

true

An obligate anaerobe can be cultured in a candle jar

false

Agar is a useful compound in the microbiology lab because it is an excellent nutrient for bacteria

false


A selective medium can be formulated either by including inhibitory chemical substances or by leaving out a single crucial nutrient

true

In spectrophotometry, 40% light transmission is the same thing as 60% absorbance of light

true

Prokaryotic cells are diploid

false


A chemical is reported to inhibit bacterial replication. Bacterial cells are placed in medium with all nutrients necessary for replication. The chemical is added to the culture, and after a half hour an extract of the DNA is prepared. A significant percentage of the DNA is in pieces about 1000 to 2000 bases in length. The results are consistent with the chemical blocking the function of DNA ligase

true

Pyrimidine dimers are severe mutations because cells have few mechanisms for repairing them

false

Most bacteria have a natural ability to take up DNA from their environment

false

The structure of DNA explains both its ability to encode genetic information and the way in which it is copied during cell reproduction

true

DNA, which is negatively charged, wraps around positively charged histones as part of the packaging of eukaryotic chromosomes

true

Bidirectional replication means that each strand of a DNA molecule is replicated in the opposite direction from the other

false

The phenotype of an organism reflects only part of its genotype

true

In generalized transduction, viruses carry random DNA sequences from one cell to another

true

DNA polymerase III participates in the dark repair mechanism of DNA mutation repair

false

Gene therapy for human genetic diseases has not been successful yet

false

Reverse transcriptase synthesizes a DNA molecule from an RNA template

true

The goals of recombinant DNA technology include production of new organisms with useful combinationsof traits

true

Restriction enzymes are useful only on synthetic DNA's

false

Under ideal conditions, the number of DNA molecules produced during PCR increases exponentially

true

Sticky-end fragments generated by EcoRI will hydrogen bond to any other sticky-end sequence

false

A collection of bacterial clones each of which contains a portion of the gene sequences of an organism is known as a microarray of that organism's genome

false

Southern blotting is a technique that can be used to identify microbes that cannot be cultured

true

DNA fingerprinting produces a unique banding pattern of DNA fragments for comparison with other DNA samples

true

A subunit vaccine is prepared by extensive manipulation of the genome of the pathogen

false

Removal of microbes from the skin is antisepsis

true

Some viruses are inactivated by the same chemical or physical agents that damage cytoplasmic membranes

true

Refrigeration is an effective germicidal method

false

Antimicrobial agents usually work best at high temperatures and high pH levels

false

The phenol coefficient is one of the most widely used measurements of an antimicrobial agent's effectiveness

false

No chemical or antimicrobial agents inactivate prions

false

The decimal reduction time is the time required to kill all the microbes in a given sample

false

Slow freezing is more damaging to microbial cells than quick freezing

true

The pH conditions under which a chemical disinfectant is used do not alter its efficacy

false

By themselves, soaps have only degerming activity, not antimicrobial activity

true