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64 Cards in this Set
- Front
- Back
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Medium of a starch hydrolysis test?
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Starch agar
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Substrate of starch hydrolysis
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Starch
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Bacterial enzyme of starch hydrolysis?
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Amylase, an exoenzyme
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End product of starch hydrolysis?
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Glucose, maltose
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Positive appearance of starch hydrolysis?
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Clear zone formed around colony
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Negative appearance of starch hydrolysis?
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No clear zone around colony
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Reagent of starch hydrolysis?
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Grams iodine
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Purpose of starch hydrolysis?
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to determine whether bacteria hydrolyzes starch
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Purpose of ONPG test?
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to determine whether bacteria express beta galactosidace
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Medium of ONPG test?
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ONPG tablet
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Substrate of ONPG test?
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Chemical Analong (a look alike of lactose)
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Bacterial enzyme of ONPG?
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Beta galactosidase, endoenzyme
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End product of ONPG test?
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O-nitrophenol (yellow color)
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Positive appearance of ONPG test?
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Yellow
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Negative appearance of ONPG test?
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Clear
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Medium of Indole test?
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Tryptone broth
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Substrate of Indole test?
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Tryptophan, an amino acid
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Bacterial enzyme of Indole test?
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Tryptophanase
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End product of Indole test
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Indole
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Purpose of Indole test?
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to determine indole production
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Positive appearance of Indole test?
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cherry red color on top
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Negative appearance of indole test?
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pale yellow
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Reagent for indole test?
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Kovac's reagent
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Medium for MR test?
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MRVP broth
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Substrate for MR test?
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Glucose
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Bacterial enzyme for MR test?
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fermentation, endoenzymes
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End product for MR test?
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Mixed acids
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Positive appearance for MR test?
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Red < 4.4
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Negative appearance for MR test?
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Yellow > 4.4
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pH indicator of MR test?
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Methyl Red
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Reagent for MR test?
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Methyl Red
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Medium for VP test?
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MRVP broth
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Substrate for VP test?
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Glucose
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Bacterial enzyme for VP test?
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Fermentation, endoenzymes
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Product of VP test?
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Acids (24 hrs.) Acetoin (48 hrs.)
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Positive appearance of VP test?
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Red
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Negative appearance of VP test?
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Copper
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Reagent of VP test?
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VP reagents 1 and 2
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Medium of Citrate test?
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Simmons Citrate Agar
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Substrate of Citrate test?
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Citrate/citric acid
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Bacterial enzyme of Citrate test?
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Citrase
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Product of Citrate test?
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Alkaline products
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Purpose of Citrate test?
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to determine whether bacteria can utilize citrate as its sole carbon source
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Positive appearance of Citrate test?
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Blue color, or green with growth
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Negative appearance of Citrate test?
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No growth, green color
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pH indicator of Citrate test?
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Bromthymol blue
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Citrate
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Chemically defined medium: the exact percent of composition is known
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Nutrient Agar
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Complex undefined medium- exact percent composition unknown
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Agar
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Complex carbohydrate that is not a nutrient, melts at 100 degrees C, stays liquid at 43
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Medium of OF Glucose test?
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OF medium
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Substrate of OF Glucose test?
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Glucose
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Bacterial enzyme of OF Glucose test?
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endoenzymes
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Product of OF Glucose test?
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Acid
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Purpose of OF Glucose test?
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to determine whether bacteria oxidize and or ferment glucose
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Positive appearance of OF Glucose test?
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Yellow
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Negative appearance of OF Glucose test?
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Blue or Green
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pH indicator of OF Glucose test?
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Bromethyl Blue
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Medium for PR broth
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PR (carbohydrate) broth
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Substrate for PR broth?
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Glucose or Lactose
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Bacterial enzyme for PR broth?
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Endoenzyme
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Product of PR broth?
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Acid +/- gas, alcohol
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Positive appearance of PR broth
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Yellow and turbidity
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Negative appearance of PR broth?
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Pink/magenta or red and turbid
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pH indicator of PR broth?
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Phenol Red
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