Microbiology - Parasitology 2 - Osler

Front 1

name the filaria

Back 1

a. Wuchereria bancrofti (90% of lymphatic filariasis. Sheathed & nuclei stop short of the tail)

b. Brugia malayi (10% lymphatic filariasis. Sheathed & 2 small dysmorphic nuclei in the tail)

c. Onchocerca volvulus (NOT SHEATHED)

d. Loa loa (Sheathed. normal nuclei through tip of tail)

Front 2

Which filaria is not diagnosed by blood but by skin snip?

Back 2

Oncocerca

Front 3

Back 3

African trypanosomiasis
(2 types: t. brucei rhodensiense (E Africa) vs gambiense (W Africa))

Has anterior flagella and posterior TERMINAL kinetoplast

Front 4

Back 4

American trypanosomiasis
= Trypanosoma cruzi

C- shaped
very dark large subterminal kinetoplast

Front 5

Back 5

Leishmania
Amastigotes with kinetoplasts in macrophage

Front 6

What parasite is transmitted by the tsetse fly?

Back 6

African trypanosomiasis

(T.b. rhodensiense & gambiense)

Front 7

What parasite is transmitted by the reduvid bug?

Back 7

American trypanosomiasis
= Trypanosoma cruzi
= Chagas disease

Front 8

What parasite is transmitted by the sandfly?

Back 8

Leishmania

Front 9

What diseases are transmitted by the Ixodes tick?

Back 9

Babesia
Lyme
Ehrlichia
Anaplasma

Front 10

What diseases are transmitted by the Aedes aegypti mosquito?

Back 10

Yellow fever
Dengue fever

Front 11

Back 11

P. falciparum
banana gametocytes

Front 12

Back 12

P falciparum
doubly infected cells

Front 13

Back 13

P falciparum
Applique form

Front 14

Back 14

Babesia
organisms are smaller (< one fifth RBC) than falciparum (~1/3) and have more variable shapes

Front 15

Back 15

Plasmodium ovale
FIMBRIATED RBC

Front 16

Back 16

P ovale (fimbriated RBC & Schuffners dots)

Front 17

Back 17

P vivax
RBCs are ENLARGED
Schuffner's dots
pigment

Front 18

Back 18

P vivax
has most merozoites per schizont (10-20)

Front 19

What region is spared by P vivax?

Back 19

West Africa.
Evolved to be Duffy a-/b-, and duffy is the receptor

Front 20

What malaria types have a latent form in the liver that must be treated?

Back 20

P vivax
P ovale

Front 21

What malaria classically shows infection in larger RBCs

Back 21

P vivax

Front 22

What malaria has the most pigment

Back 22

P malariae

Front 23

What malarias show Schuffners dots

Back 23

P vivax
P ovale

Front 24

Back 24

P malariae
Chunky brown pigment
8-12 merozoites/ schizont
normal sized RBC

Front 25

Back 25

P malariae
BAND FORM unique

Front 26

# of merozoites per schizont

Back 26

P vivax: 10-20

P malariae: 8-12

P ovale: 6-14

Front 27

Major nematode / roundworms

Back 27

– Enterobious vermicularis (pinworm)
– Tricuris triciuria (whipworm)
– Ascaris lumbricoides
– Hookworms
– Strongyloides stercoralis

Front 28

Back 28

Pinworm eggs
Enterobius vermicularis
flattened on one side
50-60um x 20-40um

Front 29

"bilaterally symmetric egg and bilaterally symmetric name"!

Back 29

Trichuris trichiura
Whipworm
2 opercula on opposite sides
infective after 3 weeks in the soil
50um x 20um

Front 30

Back 30

Ascaris lumbricoides
gross!
30cm

Front 31

Back 31

Ascaris lumbricoides
Fertilized egg (round)
Ruffled outer surface ~ bark ~ LUMBER

Front 32

Back 32

Ascaris lumbricoides
Unfertilized egg (elongated)

Front 33

Back 33

Ancylostoma duodenale
Hookworm
TEETH!!

Front 34

Back 34

Necator americanus
Hookworm
No teeth. Cutting plates.

Front 35

Back 35

Hookwork egg
Thick lucent smooth shell
can't speciate

Front 36

Worm most associated with eosinophilia

Back 36

strongyloides

Front 37

only worm that can reproduce repeatedly in the host and autoinfect, or can live entirely in the soil

Back 37

strongyloides

Front 38

larvae of hookworm or strongyloides?

Back 38

hookworm larvae
-LONG buccal canal
- no genital primordium

Front 39

larvae of hookworm or strongyloides?

Back 39

strongyloides larvae
-SHORT buccal canal
- prominent genital primordium

Front 40

Back 40

Schistosoma mansoni
LATERAL SPINE

(manson the murderer)

Front 41

Back 41

schistosoma haemotobium
TERMINAL spine

(looks like you can draw blood with it)

Front 42

Which schistosoma are GI parasites? bladder?

Back 42

S. mansonia and japonicum are GI

S. haemotobium is bladder

Front 43

Chronic infection with S mansoni?

S haemotobium?

Back 43

S mansoni & japonicum: eggs get stuck in liver, cause fibrosis, and can lead to cor pulmonale

S haem: Bladder issues, increased salmonella UTIs, and SCC!

Front 44

Back 44

schistosoma japonicum
rounder egg than the others, with a small lateral spine or no spine at all

Front 45

what fluke is strongly associated with cholangiocarcinoma?

Back 45

clonorchis sinensis

Front 46

Back 46

clonorchis sinensis
WIDE operculum
little spine at end
30um

Front 47

Back 47

Fasciola or fasciolopsis
Top can Pop!
large eggs

Front 48

100um

Back 48

Paragonimus egg
looks like clonorchis but is 3x the size
Large operculum

Front 49

Back 49

Diphyllobothrium latum egg
60um
operculum
thin shell
spine

Front 50

Back 50

Taenia solium
cysticercus

Front 51

Back 51

taenia egg
Round
radial striations
hooklets in center!

Front 52

Back 52

Pollen!
has radial striations like taenia but no hooklets

Front 53

Back 53

taenia solium

Front 54

Back 54

taenia saginatium
>15 uterine branches

Front 55

Back 55

Taenia solium
7-12 primary uterine branches

Front 56

liver

Back 56

hydatid cyst from echinococcus
protoscoleces

Front 57

Back 57

hymenolepis egg
round
space between embryo and shell

Front 58

Back 58

Pediculus humanus
Body / head louse

One pair of antennae, each with five segments
Six legs project from the fused segments of the thorax
these legs are short and terminate with a single claw and opposing "thumb".

Front 59

Back 59

Pthirus pubis
Pubic lice

ROUND body
back legs are THICKER and have claws

Front 60

Which schistosoma eggs are acid fast?

Back 60

Mansoni & japonicum; hematobium is negative on AFB

Front 61

submucosa of body of stomach

Back 61

anisakis

fish nematode
Asia

**distinctive Y-shaped lateral cords!