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18 Cards in this Set
- Front
- Back
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Name the 2 obligate human mycobacteria pathogens.
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M. tuberculosis
M. leprae |
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How does the interferon-gamma release assay work?
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The patients lymphocytes are incubated with TB antigens. If the patient has been exposed to TB in the past, the IFN-gamma will be increased. IFN-gamma is detected by ELISA or by in-situ staining and counting cells.
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What are the solid culture media for mycobacteria?
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Lowenstein-Jensen
Middlebrook 7H11 |
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DNA sequencing for mycobacterial identification targets what? (3)
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16S rRNA gene
Hsp65 rpoB |
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What is the most common drug resistance in M. tuberculosis?
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Isoniazid (9.1% of US isolates in 1991)
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What genes are identified in isoniazid reisstance?
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katG: a catalase/peroxidase, probably responsible for transforming isoniazid to an active drug
inhA: involved in mycolic acid synthesis, probably a direct target of isoniazid action |
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At what CD4 count does TB become a reactivation disease?
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CD4 = 500
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Which mycobacteria is niacin producing and nitrate reducing?
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M. tuberculosis
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Which mycobacteria is niacin and nitrate negative?
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M. avium complex
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Which mycobacteria is niacin negative and nitrate positive?
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M. kansasii
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Name 3 rapid growers. What does it mean to be a rapid grower?
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M. fortuitum
M. chelonae M. abscessus Grows in 7 days or less |
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Which mycobacterium is associated with nail salons and foot baths?
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M. fortuitum
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In M. leprae infection cellular response results in ____ disease while humoral response results in ____.
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In M. leprae infection cellular response results in LIMITED (TUBERCULOID LEPROSY) disease while humoral response results in LEPROMATOUS LEPROSY.
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Which is the "tap water" chromogen?
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M. gordonae
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What special stains can be used to detect mycobacteria? (2)
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Acid fast stain
Auramine-rhodamine fluorochrome stain NOTE: on Wright-Geisma appear as ghosts |
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This mycobacterium is characterized by broad bacilli with cross banding.
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M. marinum
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This mycobacterium is characterized by bacilli with a shepherd's crook.
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M. kansasii
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This mycobacterium shows cording.
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M. tuberculosis
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