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31 Cards in this Set
- Front
- Back
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Msa |
Selective (High concentration of salt) - disease causing staphylococci ferments releasing acid turning medium around the colony yellow - non disease causing bacteria doesn't change color of medium - staphylococcus aureus (yellow) - staphylococcus epidermis (growth) - e coli (no growth) |
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Emb |
*Selective (inhibiting the growth of gram positive bacteria but allowing gram negative bacteria to grow) * differential nonlactose, lactose, and heavy lactose fermenters. - Checks for presence of e coli - production of acid will cause pink to metallic green color - e coli (metallic green) e. Areogens
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Macconkey |
- selective (inhibiting the growth of gram positive bacteria but allowing gram negative to grow including coliforms) -differential (non lactose and lactose fermenters) Lactose fermenter will appear pink
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Tntc |
More than 300 |
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Tftc |
Less than 30 |
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Cyano"bacteria" |
- anabaena - oscillatoria - rivularia |
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Algae |
Spirogyra Diatoms Peridinium Chlamdomonas |
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Protozoas |
Paramecium Amoeba proteus |
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Negative |
Nigrosin |
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Simple staining |
Methylene blue Safrinin |
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Capsule staining |
Safrinin or crystal violet |
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Gram staining |
Crystal violet |
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Acid fast staining |
Methylene blue |
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Endspore |
Malachite green |
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Blood agar |
Sheep blood Some bacteria grows some breaks down |
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Alpha hemolysis |
Looks like a "halo" narrow growth |
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Beta hemolysis |
Clear around bacteria starts narrow becomes wider |
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Gamma hemolysis |
No change in blood around bacteria |
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Halophiles |
Grow at higher salt concentrations |
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Facultative halophile |
Bacteria that grows in low salt and high salt |
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Obligate halophile |
Only grow presence of high salt |
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Obligate aerobe |
Rely on oxygen |
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Facultative anaerobe |
Organism that can grow with or without oxygen |
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Obligate anaerobe |
Can't grow with oxygen |
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Aerotolarant anaerobes |
Don't use oxygen but can grow in oxygen |
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Zone of inhibition |
Clear circular area around the disc |
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Complex medium |
Extracts are not chemically definable |
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Chemically defined medium |
Puré organic or inorganic compounds added in exact amounts |
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Enriched medium |
Specific nutrients added to support growth of fastidious organisms |
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Fastidious |
Organisms the need unusual or complex nutrient mixtures to grow |
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Spectrophotometer |
An instrument that can be used to determine bacterial cell numbers |
