Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
12 Cards in this Set
- Front
- Back
What is the purpose of this staining methodology?
|
To distinguish between Gram-Positive and Gram-Negative bacteria
|
|
|
Name the most critical step in the gram staining that brings the difference in the final staining of G+ and G- bacteria?
|
Decolorization
|
|
|
The mordant of Gram staining is _________________
|
The mordant of Gram staining is Iodine.
|
|
|
The decolorizing agent of Gram staining is ________________
|
The decolorizing agent of Gram staining is ethyl alcohol.
|
|
|
The second stain of Gram staining is _____________
|
The second stain of Gram staining is Safranin
|
|
|
What is the difference between Gram -Positive and Gram-Negative?
|
Gram positive vs Gram negative:
GRAM POSITIVE: (1) Those bacteria which are not decolorized are stained PURPLE (2) have THICK walls which are composed of proteins and cross-linked mucopeptides. GRAM NEGATIVE: (1) The decolorized bacteria that take up safranin are stained RED. (2) cell walls are complex multilayered structured that contain lipids in addition to proteins and mucopeptides. |
|
|
What is Gram-Positive and describe its appearance.
|
GRAM POSITIVE:
(1) Those bacteria which are not decolorized are stained PURPLE (2) have THICK walls which are composed of proteins and cross-linked mucopeptides. |
|
|
What is Gram-Negative and describe its appearance
|
GRAM NEGATIVE:
(1) The decolorized bacteria that take up safranin are stained RED. (2) cell walls are complex multilayered structured that contain lipids in addition to proteins and mucopeptides. |
|
Is this Gram-Positive or Gram-Negative bacteria?
|
Gram Positive
|
|
Is this Gram-Positive or Gram-Negative?
|
Gram-Negative
|
|
|
List 5 Gram-Positive bacteria
|
(1) Bacillus thuringiensis
(2) Staphylococcus aureus (3) Streptococcus agalacitiae (4) Streptococcus lactis (5) Streptococcus pneumoniae |
|
|
List 5 Gram-Negative bacteria
|
(1) Enterobacter aerogenes
(2) Escherichia Coli (3) Klebsiella pneumoniae (4) Proteus Vulgaris (5) Rhodospirillum Rubum (5) |
