Related Essays

  • Gram Staining In Microbiology

    Observe each slide under the microscope, determining whether the bacteria are gram-positive which is stained purple or gram-negative which is stained red. ...

  • Microbiology Case Study: Toxic Shock Syndrome

    The color remains in the bacterium’s cell wall after decolorization and a counter-stain process; thus, resulting in a positive Gram

  • Gram Stain Lab Report

    95% ethanol is used as the decolorizing agent, which will wash the crystal violet from Gram-negative bacteria, and a final counterstain to stain bacteria tha...

  • Microorganism: Lab Report

    Week 3 of lab, we received our unknown microorganisms. That week we performed a differential staining to determine whether our unknown has a gram-negative en...

  • Essay On Gram Staining Procedure

    However, when washed with alcohol, bacteria with thicker cell walls containing peptidoglycans tend to retain these molecules and remain violet in color, thus...

  • Gram Staining Experiment

    If the bacteria stains positive it will retain a purple/blue color. If the bacteria stains negative, the bacteria will not retain the purple/blue color, but ...

  • Microscope Lab Report

    Most of the objects, such as bacteria are transparent and light can pass through them. This bacterium was stained, the light was reflected and the bacteria w...

  • Gram-Negative Bacteria Lab Report

    Gram-negative bacteria cells do not have a thick peptidoglycan layer and are not able to retain the violet dye. However, Gram-negative cells can pick up the ...

  • Biology Lab Report Essay

    Test Purpose Observations Result Gram Staining The purpose of this test is to determine the shape as well as the nature of the bacteria’s cell wall by dis...

  • Gram Staining Test

    Gram staining was used to distinguish the unknown microbes between gram-positive microbes from gram-negative microbes. Gram-positive microbes contain a thick...

  • Shuffle
    Toggle On
    Toggle Off
  • Alphabetize
    Toggle On
    Toggle Off
  • Front First
    Toggle On
    Toggle Off
  • Both Sides
    Toggle On
    Toggle Off
  • Read
    Toggle On
    Toggle Off
Reading...
Front

Card Range To Study

through

image

Play button

image

Play button

image

Progress

1/6

Click to flip

Use LEFT and RIGHT arrow keys to navigate between flashcards;

Use UP and DOWN arrow keys to flip the card;

H to show hint;

A reads text to speech;

6 Cards in this Set

  • Front
  • Back
What is Negative Staining ?
Negative stain (definition) – does not stain bacteria but colors the background instead.

background = dark
bacteria = clear
The only staining procedure in which neither heat fixing nor strong chemicals are used is ______________________?
Negative Staining.

Since there is no heat fixation-the morphology of bacteria is not altered
Negative stains include _____________________?
Negative stains include:
(1) Nigrosin
(2) india ink
(3) congo.
Why are bacterial cells not stained with this dye?
Bacteria stained with negative staining that also have the same negative charge REPEL the dye, and maintain a clear appearance.
What is the purpose of negative staining?
FUNCTION:
(1) provides a background to view bacteria.

PURPOSE:
(2) ****It can be used for the identification of certain bacteria (spirochetes) or structures (bacterial capsules), spores and difficult to stain bacteria.
Name two important diseases caused by spirochetes?
(1) Syphillis
(2) Lyme Disease