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13 Cards in this Set

  • Front
  • Back
What stain is used for a Simple Stain?
And what color is the microbe/background
Methylene Blue
colorless background
blue microbe
What stains can you do with
Methylene Blue?
Simple Stain
Acid-Fast Stain
What stain do you use for a Negative stain?
Microbes?
Organism and bacground?
Congo Red(Micrococcus) or Nigrosin (Bacillus)
microbe is unstained
background is stained
What stains can you do with Safranin?
Gram- Stain
Spore Stain
How do you do a Gram -Stain?

What is a gram stain based on?
(cell wall structure)
Crystal violet
Iodine(mordant)
Decolorizer
Safranin
Gram Stains React with what two types and what color do they turn?
Gram Positive: Purple/violet/blue
Gram Negative: Red/Pink
On What can DECOLORIZING AGENTS be used on?
Decolorizer (gram stain)
Acid-alcohol (acid-fast)
Capsule Stains?
Microbe?
Background?
Capsule is clear
Microbe is stained
Background is stained
STEAMING METHODS are used for what two stains?
Acid Fast Stain
Spore Stain
Spore Stain Dyes?
Microbe Color?
Malachite Green and Safranin
Spore stain- GREEN
Non spore stain- RED/PINK
Alternate Names for:
Acid-Fast
Spore-Stain
ACID-FAST
Ziehl-Neelsen
SPORE STAIN
Schaeffer-Fulton
HOW DO YOU DO AN
ACID FAST STAIN?
Microbe color?
carbol fuchsin
acid-alcohol
Methylene Blue
POS-red/pink NEG- blue
Counter Stains?
Safranin (gram & spore stains)
Methylene Blue (acid-fast)