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22 Cards in this Set
- Front
- Back
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culture media
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have nutrients to support the growth of bacteria
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macronutrients
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C, H, O, N
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micronutrients
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trace elements
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auxotroph
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requires nutritional supplement for growth
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prototroph
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requires no organic nutrients
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example of synthetic media
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Burk's media
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example of rich media
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nutrient broth
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agar concentration
broth |
0%
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agar concentration
semisolid |
.4%
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agar concentration
solid |
2%
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general media
function |
provides basic needs for bacterial growth
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general media
examples |
Tryptic Soy Agar
Nutrient Agar |
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selective media
function |
used to suppress growth of unwanted microorganisms & favor the growth of desired organisms
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selective media
examples |
MacConkey
Mannitol Salt Colistin Nalidixic |
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differential media
function |
distinguish colonies of desired organisms from other colonies growing on same plate
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differential media
examples |
MacConkey
Mannitol Salt Blood Agar |
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MacConkey
stains for what? |
lactose fermentation
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Mannitol Salt
stains for what? |
mannitol fermentation
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autoclave
definition |
sterilization
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autoclave conditions
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15 minutes
15 psi 121*C |
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sterilization
definition |
kills all forms of life
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aseptic technique
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combination of lab methods used to keep the worker, the work area, and the desired organisms being cultured free of bacterial contamination
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