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23 Cards in this Set
- Front
- Back
- 3rd side (hint)
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What nutrients are in Chocolate Agar and what does it grow?
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Heme from lysed blood cells, TSA, NAD for H Influenzae.
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What does sheep blood agar differentiate?
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Amount of hemolysis, BAG from most to no hemolysis
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Agar that diffentiates fermenting and non-fermenting organisms.
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MacConkey Agar (pink when positive)
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What does MacConkey Agar grow?
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negative rod bacteria
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detects streptococcus pyogenes and micrococcus
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Bactracin Disc sensitivity
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zone of inhibition around an antibiotic
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blackening of the medium due to precipitation of iron salts
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bile esculin to detect hydrolysis of esculin
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test specific for group B streptococci
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CAMP
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streak a B-lysing staph aureus on BAP and perpendicular unknown
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What is the catalase test?
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drop hydrogen peroxide on organism and see if it bubbles
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what can make a false positive catalase test?
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blood from BAP with the organizm because RBCs contain catalase
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positive citrate test
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green to blue or visible growth indicates utilization of citrate as a sole carbon source
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difference between bound and free coagulase
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bound - bound on cell wall and detected by slide + water + plasma; clumping is with one another
free - released by cell wall and detected by tube test; .5ml of plasma + colony will cause a fibrin clot if positive |
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test used to speciate members of th neisseria genus
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Cysteine Trypticase Agar sugar test; tests ability for the organism to ferment various sugars and sugar combinations
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gonorrheoal, meningitidis, lactamica, mucosa
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test to detect ability to degrade tryptophan
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indole test (+ if red ring)
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positive nocobiocin susceptibility test
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staphylococcus saprophyticus
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test to differentiate streptococcus pheumoniae from viridans
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optochin disc test
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+ if zone of 12 mm or more around optochin disc after 24 in 5% CO2 increase
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positive oxidase test
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purple
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indicates cytochrome c oxidases
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purpose of PYR disk test
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identifying Streptococcus pyogenes from other beta hemolytic organisms
identifying enterococcus from other gamma hemolytic organisms |
PYR disk + water + PYR reagent should turn red
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causes red color in PYR test
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beta naphtylamide
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How is H. influenzae detected?
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satellite pheomenon; grown across a streak of S aureus which provides HI with NAD and hemin from RBC lysis
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Test on BAP
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What will grow in 6.5% NaCl broth?
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Enterococcus, the only group D streptococci.
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What does red and yellow mean on the TSI test?
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Red is a negative test; the organism cannot ferment and produce acid byproducts
Yellow is a positive test result and means the organism can ferment |
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What does alkaline slant with yellow butt on TSI mean?
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organism can germent glucose only; not enough glucose on slant to grow.
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What does gas bubbling and blackening of the agar mean in a TSI test?
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bubbling means organism produces gas during fermentation;
black means it produces H2S |
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