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23 Cards in this Set
- Front
- Back
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Paramecium
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What is this image?
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Volvox
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What is this image?
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Anabaena
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What is this image?
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Spirogyra
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What is this image?
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Euglena
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What is this image?
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How many Eukaryotic cells are in the body?
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10 to the 14th power
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How many prokaryotic cells are in the body?
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10 to the 13th power
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What is the name of the wire loop that you use to spread bacteria?
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inoculating loop
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what is the name of the straight wire that you use to poke bacteria?
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Inoculating wire
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what id the name of the dish that you put bacteria into to allow to spread?
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Petri Dish
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Small tubular syringe that sucks up water or liquid?
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pipette
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Reason for the hanging drop technique (slide)?
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A way to view the microbes and their motility and movement.
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How to calculate nutrient agar
23g = x ---- ----- 1000ml 850ml |
20g of nutrient agar
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steps to make hanging drop slide
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1) get a slide
2) with atoothpick and vaseline, mark around in a circle with the vaseline on the slide 3) place one drop of liquid in center of circle 4) cover droplet and circle of vaseline with coverlet to seal closed |
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Reason for using Aseptic transfer technique
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Since bacteria is everywhere, you want to start with a pure culture, therefore aseptic technique needs to be used
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Reason for oil immersion?
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to be able to magnify and clearly see the microbes over 400x
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Simple stain technique
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1) add water onto slide
2) flame loop 3) place loop into bacteria 4) add bacteria to slide 5) flame slide over fire quickly 6) add 1 drop of methylene blue to slide 7) let dry 2 minutes 8) observe under microscope |
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reason for nutrient broth?
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to keep the microbes in a more natural setting
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reason for the use of a negative stain?
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to outline the microbe with stain and not heat distort the microbe
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reason for TSA agar?
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used as an inital growth medium for microbes
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Trypanosoma brucei
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what is this bacteria?
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micrococcus luteus
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what is the name of this bacteria?
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Escherichia Coli
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what is the name of this bacteria?
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