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28 Cards in this Set
- Front
- Back
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Inoculate
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Add small amount of microbe to media (food) to obtain microbial growth
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Incubate
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Place in an incubator at a set temperature in order to get microbe to grow
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Autoclave
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method used to kill microbes using moist heat and parameters of 121C at 15 psi for 15 min
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body temp
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37C
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room temp
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25C
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refrigerator temp
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4C
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Aseptic technique
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measures we use to prevent contamination and preserve a pure culture
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What are the 4 types of organic molecules?
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proteins, carbohydrates, nucleic acids, lipids
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Example of Disaccharides
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maltose (glucose-glucose)
sucrose (glucose-fructose) lactose (glucose-galactose) |
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Example of Polysaccharides
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glycogen
starch cellulose |
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Staphylococcus aureus
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G+ Bacteria, coccal, facultative anaerobe
Halophile (salt loving) Grape like clusters |
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Micrococcus luteus
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G+ Bacteria, Spherical, obligate aerobe.
Found in human mouth, mucosae, oropharynx and upper respiratory tract. |
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Bacillus subtilis
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G+ Bacteria, bacillus, obligate aerobe
endospore |
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How are agar plates positioned when incubated? Why?
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upside down. Prevents condensation form dripping onto plate.
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How are agar plates positioned when disposed of?
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right-side up
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What is the proper labeling of agar plates?
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Organism Name
Media, Temp Date, Lab # Your Name |
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Name the types of flagella.
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A. Monotrichous
B. Lophotrichous C. Amphitricous D. Peritrichous |
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Name the bacterial shapes.
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A. Bacilli
B. Stretococci C. Staphylococci D. Diplococci E. Spirochete F. Club Rod G. Filamentous H. Streptobacilli |
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Study these morphological terms.
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Name and describe the Shapes, Margins, and Elevations terms.
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Amino acids are held together by what kind of bond?
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peptide bond
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A long chain of amino acids is called?
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polypeptide
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Describe DNA and RNA base pairing.
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DNA: Adenine:Thymine, Cytosine:Guanine
RNA: Adenine:Uracil, Cytosine:Guanine |
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What are the three types of RNA?
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mRNA (provides genetic message)
tRNA (transfers amino acids to ribosome) rRNA (makes up ribosome) |
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How many hydrogen bonds does A:T have? C:G?
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A:T has 2 and C:G has 3
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DNA has a 3' hydroxyl end and 5' phosphate end. Which end are nucleotides added to during replication.
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3' end.
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Describe replication, transcription, translation,
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DNA replicated during replication, DNA is converted to mRNA in transcription, mRNA codons are used as instruction for which amino acid to use in protein synthesis.
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Identify the microorganism. What type of microorganism is it?
What type of stain was used? |
Saccharomyces cerevisiae (yeast/fungi)
Crystal violet simple stain. |
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Identify the microorganism.
G+ or G-? |
Bacillus subtilis
G+ |
