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28 Cards in this Set

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Inoculate
Add small amount of microbe to media (food) to obtain microbial growth
Incubate
Place in an incubator at a set temperature in order to get microbe to grow
Autoclave
method used to kill microbes using moist heat and parameters of 121C at 15 psi for 15 min
body temp
37C
room temp
25C
refrigerator temp
4C
Aseptic technique
measures we use to prevent contamination and preserve a pure culture
What are the 4 types of organic molecules?
proteins, carbohydrates, nucleic acids, lipids
Example of Disaccharides
maltose (glucose-glucose)
sucrose (glucose-fructose)
lactose (glucose-galactose)
Example of Polysaccharides
glycogen
starch
cellulose
Staphylococcus aureus
G+ Bacteria, coccal, facultative anaerobe
Halophile (salt loving)
Grape like clusters
Micrococcus luteus
G+ Bacteria, Spherical, obligate aerobe.
Found in human mouth, mucosae, oropharynx and upper respiratory tract.
Bacillus subtilis
G+ Bacteria, bacillus, obligate aerobe
endospore
How are agar plates positioned when incubated? Why?
upside down. Prevents condensation form dripping onto plate.
How are agar plates positioned when disposed of?
right-side up
What is the proper labeling of agar plates?
Organism Name
Media, Temp
Date, Lab #
Your Name
Name the types of flagella.
A. Monotrichous
B. Lophotrichous
C. Amphitricous
D. Peritrichous
Name the bacterial shapes.
A. Bacilli
B. Stretococci
C. Staphylococci
D. Diplococci
E. Spirochete
F. Club Rod
G. Filamentous
H. Streptobacilli
Study these morphological terms.
Name and describe the Shapes, Margins, and Elevations terms.
Amino acids are held together by what kind of bond?
peptide bond
A long chain of amino acids is called?
polypeptide
Describe DNA and RNA base pairing.
DNA: Adenine:Thymine, Cytosine:Guanine
RNA: Adenine:Uracil, Cytosine:Guanine
What are the three types of RNA?
mRNA (provides genetic message)
tRNA (transfers amino acids to ribosome)
rRNA (makes up ribosome)
How many hydrogen bonds does A:T have? C:G?
A:T has 2 and C:G has 3
DNA has a 3' hydroxyl end and 5' phosphate end. Which end are nucleotides added to during replication.
3' end.
Describe replication, transcription, translation,
DNA replicated during replication, DNA is converted to mRNA in transcription, mRNA codons are used as instruction for which amino acid to use in protein synthesis.
Identify the microorganism. What type of microorganism is it?
What type of stain was used?
Saccharomyces cerevisiae (yeast/fungi)
Crystal violet simple stain.
Identify the microorganism.
G+ or G-?
Bacillus subtilis
G+