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26 Cards in this Set

  • Front
  • Back
The capsule creates a mucoid phenotype. This is Klebsiella pneumoniae
The appearance of the colonies on this blood agar plate is due to...
Elek plate. It tests fot toxin presence for C. diphtheriae
The name of this plate is
Eosin Methylene Blue (EMB) Agar - is is demonstrating the green metallic sheen of E. coli
What is the name of this agar and what is the enteric shown growing on it?
What is an Eosin Methylene Blue (EMB) Agar and what does it contain?
Contains eosin & methylene blue to inhibit Gram +ve bacteria. Eosin also acts as an indicator for fermentation. Lacotse & sucrose are the sugars
Which E. coli strain is non-motile, a late/non lactose fermentor and anearobic? and what other species is it very closely related to?
Enteroinvasive E. coli is non-motile and a late/non lactose fermentor and anaerobic similiar to Shigella
Which of these enterobacteriacea is NON-motile at 36 degrees? Shigella, Serratia, Proteus or citrobacter?
Shigella is non motile at 36 degrees. So is klebsiella and Yersinia
Which enterobacteriaceae are non motile at 36 degrees?
Shigella, Klebsiella and Yersinia (which is motile at 22 degrees)
Which of the following is NOT a lactose fermentor? Klebsiella, E. coli, Salmonella, Citrobacter, Serratia?
Salmonella is non-lactose fermenting. Both serratia and Citropbacter are late lacose fermentors.
Which enterobacteriaceae species is both motile at 36 degrees AND Voges-Proskaur positive?
Which enterobacteriaceae are hydrogen sulfide positive?
Salmonella, Proteus, Citrobacter and Edwardsiella
What category of bacteria ferment glucose, are oxidase negative and (mostly) reduce nitrate to nitrites?
The Enterobacteriaceae
Except for Shigella, all clinically relevant enterobacteriaceae are catalase __________
What is listeriolysin O?
It is a virulence factor for listeria monocytogenes which allows it to escape from a phagosome before lysosomal fusion, allowing the bacteria to survive within phagocytes!
What are the components of the diptheria toxin?
Fragment A - inhibits protein synthesis
Fragment B - receptor binding/translocation
What is the name of the initial lesion casued by cutaneous diptheriae?
ecthyma diphtheriticum - resembles several other skin conditions
What are the two forms of C. diphtheriae infection?
Respiratory and cutaneous
Both respiratory and cutaneous forms of diptheriae infections can cause what other symptoms?
Myocardiopathy (20-70%)
Neurological symptoms (20-75%)
Fatty/focal necrosis of kidneys, liver or adrenals and death
What is the most commonly isolated Corynebacterium species?
C. jeikeium
What process was used to isolate L. monocytogenes from placental or other tissue?
cold enrichment- placed in nutrient broth for months at 3 degrees. Replaced by more rapid id methods
What growth requirement is specific to C. diphtheriae subspecies intermedius?
C. diptheria intermedius requires lipids for growth
C. diphtheriae and C. jeikeium are both urease ____
negative. Other corynebacterium C. ulcerans and C. pseudotuberculosis are urease positive.
Which of the following enterobacteriacaea is NOT phenyalanine deaminase positive? Proteus, Pantoea, Morganella, Providencia?
Which of the following is hydrogen sulfide positive? Enterobacter, Serratia, Edwardsiella, Klebsiella, Yersinia?
What are the hydrogen sulfide producing enterobacteriacaea?
Salmonella, Proteus (vulgaris and mirabilis), Citrobacter freundii and Edwardsiella tarda
This is C. diphtheriae and growth on Loffler medium enhances metachromatic granules. Notice also the "Chinese characters".
What bacteria is this and what growth medium will enhance the granules seen?
How does iron affect C. diptheriae toxin production?
The toxin production is inhibit in high iron environments and enhanced with iron deficiency.