Microbiology For Pathologists

Front 1

The capsule creates a mucoid phenotype. This is Klebsiella pneumoniae

Back 1

The appearance of the colonies on this blood agar plate is due to...

Front 2

Elek plate. It tests fot toxin presence for C. diphtheriae

Back 2

The name of this plate is

Front 3

Eosin Methylene Blue (EMB) Agar - is is demonstrating the green metallic sheen of E. coli

Back 3

What is the name of this agar and what is the enteric shown growing on it?

Front 4

What is an Eosin Methylene Blue (EMB) Agar and what does it contain?

Back 4

Contains eosin & methylene blue to inhibit Gram +ve bacteria. Eosin also acts as an indicator for fermentation. Lacotse & sucrose are the sugars

Front 5

Which E. coli strain is non-motile, a late/non lactose fermentor and anearobic? and what other species is it very closely related to?

Back 5

Enteroinvasive E. coli is non-motile and a late/non lactose fermentor and anaerobic similiar to Shigella

Front 6

Which of these enterobacteriacea is NON-motile at 36 degrees? Shigella, Serratia, Proteus or citrobacter?

Back 6

Shigella is non motile at 36 degrees. So is klebsiella and Yersinia

Front 7

Which enterobacteriaceae are non motile at 36 degrees?

Back 7

Shigella, Klebsiella and Yersinia (which is motile at 22 degrees)

Front 8

Which of the following is NOT a lactose fermentor? Klebsiella, E. coli, Salmonella, Citrobacter, Serratia?

Back 8

Salmonella is non-lactose fermenting. Both serratia and Citropbacter are late lacose fermentors.

Front 9

Which enterobacteriaceae species is both motile at 36 degrees AND Voges-Proskaur positive?

Back 9

Klebsiella

Front 10

Which enterobacteriaceae are hydrogen sulfide positive?

Back 10

Salmonella, Proteus, Citrobacter and Edwardsiella

Front 11

What category of bacteria ferment glucose, are oxidase negative and (mostly) reduce nitrate to nitrites?

Back 11

The Enterobacteriaceae

Front 12

Except for Shigella, all clinically relevant enterobacteriaceae are catalase __________

Back 12

positive

Front 13

What is listeriolysin O?

Back 13

It is a virulence factor for listeria monocytogenes which allows it to escape from a phagosome before lysosomal fusion, allowing the bacteria to survive within phagocytes!

Front 14

What are the components of the diptheria toxin?

Back 14

Fragment A - inhibits protein synthesis
Fragment B - receptor binding/translocation

Front 15

What is the name of the initial lesion casued by cutaneous diptheriae?

Back 15

ecthyma diphtheriticum - resembles several other skin conditions

Front 16

What are the two forms of C. diphtheriae infection?

Back 16

Respiratory and cutaneous

Front 17

Both respiratory and cutaneous forms of diptheriae infections can cause what other symptoms?

Back 17

Myocardiopathy (20-70%)
Neurological symptoms (20-75%)
Fatty/focal necrosis of kidneys, liver or adrenals and death

Front 18

What is the most commonly isolated Corynebacterium species?

Back 18

C. jeikeium

Front 19

What process was used to isolate L. monocytogenes from placental or other tissue?

Back 19

cold enrichment- placed in nutrient broth for months at 3 degrees. Replaced by more rapid id methods

Front 20

What growth requirement is specific to C. diphtheriae subspecies intermedius?

Back 20

C. diptheria intermedius requires lipids for growth

Front 21

C. diphtheriae and C. jeikeium are both urease ____

Back 21

negative. Other corynebacterium C. ulcerans and C. pseudotuberculosis are urease positive.

Front 22

Which of the following enterobacteriacaea is NOT phenyalanine deaminase positive? Proteus, Pantoea, Morganella, Providencia?

Back 22

Pantoea.

Front 23

Which of the following is hydrogen sulfide positive? Enterobacter, Serratia, Edwardsiella, Klebsiella, Yersinia?

Back 23

Edwardsiella.

Front 24

What are the hydrogen sulfide producing enterobacteriacaea?

Back 24

Salmonella, Proteus (vulgaris and mirabilis), Citrobacter freundii and Edwardsiella tarda

Front 25

This is C. diphtheriae and growth on Loffler medium enhances metachromatic granules. Notice also the "Chinese characters".

Back 25

What bacteria is this and what growth medium will enhance the granules seen?

Front 26

How does iron affect C. diptheriae toxin production?

Back 26

The toxin production is inhibit in high iron environments and enhanced with iron deficiency.