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43 Cards in this Set
- Front
- Back
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What is a cell line?
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A diploid cell culture that is created from subculturing a primary culture. They have around 50 transfers.
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What are examples of cell lines?
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human diploid fibroblasts HDF, and MRC-5
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What is an established cell line?
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Cells that continue to proliferate after at least 70 transfers, often attained from malignancies
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What are examples of established cell lines?
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Hep-2, HeLa
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Hep2 line is derived from?
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Laryngeal carcinoma
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HeLa line is derived from?
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Cervical cancer
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What is the most common cause of culture media contamination?
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Mycoplasma spp or Simian viruses
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What is the shell vial technique?
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Cell cultures within a small vial are coverslipped and inoculated and centrifuged to bring the virus in contact with the cells, the coverslip is removed after <3 days and stained with anti-viral antibodies
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Influenza histology
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No inclusions, no syncitia
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RSV histology
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Syncitia
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HSV histology
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Nuclear inclusions, syncitia, cowdry bodies
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Adenovirus histology
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Nuclear inclusions.
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CMV histology
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Nuclear and cytoplasmic inclusions
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Measles histology
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Cytoplasmic and nuclear inclusions, syncitia, Warthin-Finkeldy giant cells
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Rabies histology
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Cytoplasmic inclusions (negri bodies)
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What are the nonenveloped DNA viruses
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Parvoviridae, Adenoviridae, Picornaviridae
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What are the enveloped DNA viruses
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Herpesviridae, Poxviridae, Hepadnaviridae
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What are the nonenveloped RNA viruses
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Caliciviridae, Papovaviridae,Reoviridae
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What are the enveloped RNA viruses
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All the rest: arena, bunya, corona, flavi, filo, orthomyxo, paramyxo, toga, rhabdo
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What is HSV 1 associated with?
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gingivostomatitis, pharyngitis, keratoconjuctivitis, herpes labialis, herpetic whitlow, herpes encephalitis
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What is considered the definitive method for diagnosis of HSV1 and HSV2?
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Cell culture, although direct DNA probes are becoming more widespread
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What is the rate of growth of HSV1 and HSV2 in cell culture?
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Rapid, detectable by day 2 or 3
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What cell culture will not grow HSV1 or HSV2?
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PMK
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What is the sensitivity of a tzanck smear?
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60-70%
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How does CMV present in immunocompromised patients?
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Pneumonia, hepatitis, retinitis, or disseminated disease
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What receptor does EBV infect via?
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C3d, CD21
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What are examples of EBV serologic markers?
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anti-viral capsid antigen, VCA, anti EBNA
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HPV type: Plantar wart
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1,2
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HPV type: Common Wart
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2,1,4, 7 in fish and meat handlers
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HPV type: Flat wart
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3,10
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HPV type: oral squamous papilloma
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6,11
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HPV type: Oral focal epithelial hyperplasia (heck dz)
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13,32
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HPV type: epidermodysplasia verruciformis
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2,3,10,5,8
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HPV type: Laryngeal papillomas
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6,11
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HPV type: Condyloma acuminatum
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6,11
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HPV type: LSIL
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6,11
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HPV type: HSIL
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16,18,31,33,35
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HPV type: AIS
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16,18
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What viruses are hemadsorption positive?
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influenza A, B, and parainfluenza
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How many subtypes of H and N are there?
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15 for H, 9 for N
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What organism is associated with hemorrhagic cystitis in BM transplant patients?
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Adenovirus
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What is chronic hep B infection?
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Persistence of HbsAg after 6 months
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What is the earliest way to detect HepB infection?
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HBV DNA, 3 weeks before HbsAg
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