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23 Cards in this Set
- Front
- Back
Question
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Answer
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definition of microscopy
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technology of making very small things visible to the human eye
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milli
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10^-3 m
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micro
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10^-6 m
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nano
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10^-9 m
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wavelength
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length of light ray (lambda); = to distance between two adjacent crests or troughs of a wave
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resolution
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ability to see two items as separate and distinct units
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shorter the wavelength, the ____ resolution that can be attained
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greater
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reflection
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light strikes an object and bounces back (provides the object with color)
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transmission
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passage of light through an object
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absorption
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light rays neither pass through nor bounce off of an object, but are taken up by the object
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refraction
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bending of light as it passes from one medium to another medium of different density
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angle of refraction
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degree of bending of the light ray after it refracts
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index of refraction
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a measure of the speed at which light passes through a given material
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oil immersion prevents
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refraction of light rays; reduces amount of rays lost to lens in microscopy
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light microscopy
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use of any kind of microscope that uses visible light to make specimens observable
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compound light microscope has two lenses, called
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(1) objective lens; (2) ocular lens
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total magnification
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magnifying power of objective lens x magnifying power of ocular lens
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parfocal
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specimen remains very nearly in focus as micoscopist increases or decreases the magnification
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dark field illumination
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microscope adapted w/condenser that prevents light from being transmitted through the specimin but instead causes the light to reflect off the specimin at an angle (light object seen on dark background)
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wet mount
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drop of medium containing organisms is placed on slide and used to view living microorganisms
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smears
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microorganisms are spread onto the surface of a glass slide and used to view destroyed organisms
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heat fixation
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destroys the organisms, causes organism to adhere to slide, and alters organism to accept stains (dyes)
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