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23 Cards in this Set

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Question
Answer
definition of microscopy
technology of making very small things visible to the human eye
milli
10^-3 m
micro
10^-6 m
nano
10^-9 m
wavelength
length of light ray (lambda); = to distance between two adjacent crests or troughs of a wave
resolution
ability to see two items as separate and distinct units
shorter the wavelength, the ____ resolution that can be attained
greater
reflection
light strikes an object and bounces back (provides the object with color)
transmission
passage of light through an object
absorption
light rays neither pass through nor bounce off of an object, but are taken up by the object
refraction
bending of light as it passes from one medium to another medium of different density
angle of refraction
degree of bending of the light ray after it refracts
index of refraction
a measure of the speed at which light passes through a given material
oil immersion prevents
refraction of light rays; reduces amount of rays lost to lens in microscopy
light microscopy
use of any kind of microscope that uses visible light to make specimens observable
compound light microscope has two lenses, called
(1) objective lens; (2) ocular lens
total magnification
magnifying power of objective lens x magnifying power of ocular lens
parfocal
specimen remains very nearly in focus as micoscopist increases or decreases the magnification
dark field illumination
microscope adapted w/condenser that prevents light from being transmitted through the specimin but instead causes the light to reflect off the specimin at an angle (light object seen on dark background)
wet mount
drop of medium containing organisms is placed on slide and used to view living microorganisms
smears
microorganisms are spread onto the surface of a glass slide and used to view destroyed organisms
heat fixation
destroys the organisms, causes organism to adhere to slide, and alters organism to accept stains (dyes)