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12 Cards in this Set
- Front
- Back
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What are the six "I"'s of Microbiology? |
* Inoculation * Incubation * Isolation * Inspection * Information gathering * Identification |
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What are the two key features of microscopes? (2) |
* Magnification * Resolving Power (resolution) |
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What is Resolution? |
The ability to identify or distinguish two objects as being separate & distinct |
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What is Numerical Aperture (NA)? (4) |
* A mathematical constant derived from the physical structure of a lens * The angle of the light that is produced by refraction * A measure of the quantity of light gathered by the lens * The higher the NA, the better the resolving power |
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What is Bright Field Microscopy? (3) |
* Forms its image when light is transmitted through the specimen * White background/Dark sample * Most widely used type of light microscope |
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What is Dark Field Microscopy? (4) |
* Dark background/Light sample * Good for live specimens * Does not reveal fine internal details * Achieved with a stop (blocks all but peripheral light) to the condenser |
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What is Phase Contrast Microscopy? (4) |
* Good for live specimens * Darker areas are dense areas * Good for looking at internal structures * Black & White; 3-D looking (cool!) |
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What is Differential Interference Contrast Microscopy (DIC)? (3) |
* Detailed view of unstained, live specimens by manipulating light * Has refinements that add contrasting colors to the image * Produces extremely well defined images, vividly colored and appear 3-D |
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What is Fluorescence Microscopy? (3) |
* Uses UV light * Requires shielding for the tech * Electrons are hit with light, get excited, jump to a different shell and as they come back down emit light |
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What is a Scanning Confocal Microscope? (3) |
* Uses laser beams to scan various depths of a specimen to deliver sharp image focusing on just a single plane * You can see the surface or the middle of a cell * Can be used to visualize live, unstained cells, but is mostly used on fluorescently stained specimens |
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What is a TEM or Transmission Electron Microscope? (4) |
* Transmits electrons through the specimen (like we send light through a specimen) * Sample must be plasticized & sliced into thin sections * Must be stained with heavy metals * High voltage electricity focused by magnets |
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What is a SEM or Scanning Electron Microscope? (2) |
* Coat the outside of the specimen with gold or platinum * Put it in a special chamber and bounce electrons off to see the outside of a specimen |
