Microbiology Ch 10

Front 1

use of an organism's biochemical and metabolic pathways for industrial production

Back 1

biotechnology

Front 2

when DNA is heated from 90 to 95 the two strands ______, where the nucleotides can be identified, replicated or transcribed.

Back 2

seperate

Front 3

when the DNA is slowly cooling the strands do what? allowing the nucleotides to hydrogen bond

Back 3

come together

Front 4

this is recognizing the specific sequences of DNA and break phosphodiester bonds(when it finds it, it cuts it)

Back 4

restriction endonucleases (RE)

Front 5

most REs recognize a ______ sequence that reads the same from 5 to 3 on both strands

Back 5

palindrom
5-GAATTC-3
3-CTTAAG-5

Front 6

the enzymes can be used to ____ DNA at desired sites

Back 6

cleave

Front 7

this is used in the lab to cut DNA into smaller pieces

Back 7

restriction fragments

Front 8

restriction fragment of varying lengths are called ___ ___ ____ ____.

Back 8

restriction fragment length polymorphisms

Front 9

Some REs make a _____ cut leading to blunt ends or cut _____ leading to sticky ends

Back 9

straight
staggered

Front 10

this makes a DNA copy of RNA= cDNA

Back 10

reverse transcriptase

Front 11

this rejoins phosphate sugar bonds cut by endonuclease (glues together)

Back 11

ligase

Front 12

seperates DNA fragments based on size, rate of movement dependent on size larger fragments the movement is ____.

Back 12

gel electrophoresis
slow

Front 13

this other DNA analysis is probes base pair with complementary sequences used to detect specific sequences

Back 13

nucleic acid hybridization and probes

Front 14

reading the sequence of nucleotides in a stretch of DNA

Back 14

DNA sequencing

Front 15

way to amplify DNA, make more of

Back 15

polymerase chain reaction

Front 16

single stranded DNA can unite with other simple stranded DNA or RNA can unite with each other RNA

Back 16

hybridization

Front 17

used for diagnosing cause of infection and identifying unknown bacterium or virus

Back 17

hybridization test

Front 18

the intentional removal of genetic material from one organism and combining it with that of a different organism

Back 18

recombinant DNA technology

Front 19

with recombinant DNA the gene is inserted into a _____(plasmid, virus, cosmids) that will insert the DNA into a ____ ____.

Back 19

vector
cloning host

Front 20

these are small well characterized easy to manipulate and can be transferred into appropriate host cells through transformation

Back 20

plasmids

Front 21

have the natural ability to inject their DNA into bacterial hosts through transduction

Back 21

bacteriophages

Front 22

pseudomonas syrinage prevents?
bacillus thuringienisis?

Back 22

ice crystals
encodes an insecticide