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14 Cards in this Set
- Front
- Back
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What kind of bacteria will not stain via normal procedures?
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Bacteria that contain mycolic acid, which means that they have a higher than normal lipid content.
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mycolic acid
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complex, waxy hydrocarbon over 80 carbons in length; found in glycolipids
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What solution can penetrate the waxy cell wall of bacteria that contain mycolic acid?
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carbolfuschin + phenol
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Cells dyed with carbolfuschin (+ phenol) are termed _________ and are _____ in color.
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Cells dyed with carbolfuschin (+ phenol) are termed ACID-FAST and are RED/PINK in color.
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Cells that do not contain a mycolic acid wall and are easily decolorized by an alcohol wash are termed _________.
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non-acid-fast
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Non-acid-fast cells must be counterstained with _________.
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methylene blue
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Identify the 7-step process of acid-fast staining.
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1. Carbolfuchsin, 5 mins
2. Wash (water), 5 sec 3. Acid-alcohol, 1 min 4. Wash (water), 5 sec 5. Methylene blue, 30 sec 6. Wash (water), 5 sec 7. Blot dry |
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What do you call a bacteria in a resting state due to severe environmental conditions?
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Endospores
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Endospores are resistant to... (3)
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Heat
UV radiation Many chemicals that will kill normal bacteria |
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What conditions are required to kill endospores?
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In an autoclave, under pressure at 121°C for 15-20 minutes
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What solution is used in spore staining and how does it work?
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Malachite green penetrates the exosporium of the endospore when heat is applied.
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Why are endospores so resistant (to staining or damage)?
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Presence of exosporium
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exosporium
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extra protein coat that surrounds spore; allows endospore to be resistant to many forms of damage
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This is a spore stain.
What do the green parts represent? What do the red parts represent? |
Green = Spore
Red = Bacteria |
