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33 Cards in this Set
- Front
- Back
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Identify the 3 types of media.
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Enrichment
Selective Differential |
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What is enrichment media?
Give an example. |
It provides "extra" food, chemicals, and nutrients.
i.e. Blood agar (red one) contains blood cells. |
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What is selective media?
Give an example. |
Media with conditions that favor the growth of one bacteria over another.
i.e. SDA (dextrose) is selective for fungi. |
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Why is SDA (dextrose) selective for fungi? (2)
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These two conditions are more tolerable by fungi than bacteria:
-Dextrose (glucose) is the carbon source. -The agar has a low pH. |
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What is differential media?
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It is a media that can distinguish between different bacteria by containing substances that change color/form.
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What kind of media is blood agar? (2)
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Enrichment and differential
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How is blood agar used as differential media?
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It allows us to detect what organisms can lyse blood cells.
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What is agar?
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It is a jello-like substance used as a culture media in microbiology.
It is extracted from seaweed/kelp. |
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Identify the two kinds of selective media?
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RZA and SDA
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What is RZA media used for? (2)
How does it do its job? |
Environmental bacteria
Slow-growing bacteria It has low concentrations of nutrients so ALL organisms grow slowly. |
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What is room temperature in C?
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25° C
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What is body temperature in C?
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37° C
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Identify the 3 categories of the 3 Domain System.
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Eucarya
Bacteria Archaea |
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Identify the 5 categories of the 5 Kingdom System (old method).
Which of these are eukaryotes? Prokaryotes? |
PROKARYOTES
Monera EUKARYOTES Protista Fungi Plantae Animalia |
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Identify examples in kingdom monera.
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Bacteria
Archaea |
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Identify examples in kingdom protista.
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Algae
Protozoa |
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Identify examples in kingdom fungi. (3)
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Yeast
Mold Toadstools (mushrooms) |
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Identify examples in kingdom plantae.
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Any kind of tree, such as the dogwood, redwood, etc.
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Identify examples in kingdom animalia.
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Humans
Cats Dogs |
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Identify the kingdom that includes helminths.
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Animalia
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helminths
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a worm, especially a parasitic roundworm or tapeworm
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Identify the TOTAL magnification of the following lens: ocular.
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15x
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Identify the TOTAL magnification of the following lens: red.
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60x (4 x 15)
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Identify the TOTAL magnification of the following lens: yellow.
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150x (10 x 15)
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Identify the TOTAL magnification of the following lens: blue.
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600x (40 x 15)
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Identify the TOTAL magnification of the following lens: white.
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1500x (100 x 15)
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Which lens needs oil in order to use?
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White lens
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What is protoslo?
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solution used to slow down protists (unicellular eukaryotic organisms) and other small organisms during light microscopy procedures
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A technician incubated a blood agar plate with human oral bacteria for 24 hours at 0°C. The plate showed no growth.
What mistake did the technician make? |
The technician should have incubated the plate at 37°C, which is human body temperature.
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What is the greatest level of magnification we can achieve with the microscopes in lab?
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1500x
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Which adjustment knob is utilized only during the initial focusing procedure in microscope use?
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Coarse adjustment knob
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What purpose does the condenser serve on a microscope?
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Condense or focus light
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What lens magnifies at 15x?
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Ocular lens
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