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25 Cards in this Set
- Front
- Back
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This part of the operon makes the protein repressor:
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Regulatory Gene
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This part of the operon is the point of contact with RNA polymerase and has -10, -35 sequence
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Promoter Region
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This part of the operon has a palidrome and is recognized by the repressor protein
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Operator
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How is lactose brought into the bacterial cell?
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Via symport at the expense of hydrogen ions (with protons?)
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Glucose-lactose in the bacterial cell uses catabolite repression and uses this protein for control:
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Cyclic AMP binding protein
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If glucose is high, what happens to the level of CAP protein?
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CAP will be low and this will allow glucose to be used over lactose.
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Which two complementary regions of RNA binding form a termination hairpin?
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3 and 4
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Which two complementary regions of RNA binding form a signal to continue when the polymerase stalls?
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2 and 3
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How is expression of proteins regulated if the mRNA are polycistronic?
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Through the number of shine-delgarno sequences.
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These factors change the specificity of the bacterial RNA polymerase and cause it to form spore structures:
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Sigma factors
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These are bacterial survival structures:
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Spores (endospores)
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These complexes are deposited throughout the spore and confer heat resistance:
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dipicolonic acid- Ca++
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In infants presenting with GI symptoms and neurological symptoms, which spore forming organisms must be on the differential?
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Clostridium - in honey, botulisim
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These are suicide enzymes that directly remove abnormally placed methyl groups by accepting them onto their own structure
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Methyltransferases
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This enzyme removed cyclobutane dimers in the presence of visible light- photoreactivation
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Photolyase
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In dark excision repair, which complex incises the DNA?
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UvrABC complex
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Provide an extension and redundancy to methyltransferases by cleaving the N-glycosidic bond and removing all modified bases
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DNA glycosylases
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MUT H, L, S and U are all involved in _________.
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Mismatch repair
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Dark repair - postreplication repair involves which protein which brings DNA to areas of homology?
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RecA
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This is an inducible, error prone system
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SOS repair
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This is the abberant DNA polymerase in SOS repair that fills gaps with DNA bases at random
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UmuDC
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This happens when a nonsense codon is read by the bacteria instead of terminating the chain
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Extragenic mutation
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This occurs when the function of a protein is eliminated by one mutation and restored by another mutation at a different location
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Intragenic
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This is the most common process by which bacteria exchange genetic information
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Conjugation
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What are the two criteria for transformation?
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1. It must be high MW DNA
2. The cells must be competent |
