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25 Cards in this Set

  • Front
  • Back
Lec 4
- Nucleoid
- area of microbe where DNA
is found
- single chromosome
- Condensed by scaffolding
/DNA binding protiens
- no nuclear membrane
- Transcription & translation
at the same time... WOW
Lec 4
- Nucleoid
- Prokaryotic DNA info
- Prokaryotes are 1N Haploid
- Negative Supercoiled shape
1 - untwisted double helix
2 - easy replic/transcript
Lec 4
- Nucleoid
- Prokaryotic DNA info
- Negative Supercoiled
DNA
- who has it
- all prokaryotes have neg
supercoiled DNA
- EXCEPT Thermophiles
Lec 4
- Nucleoid
- Genome size variance in
Microbes
from .5 to 10 million base
pairs
- genome can vary up to 1000 genes in the same species
Lec 4
- Cytoplasmic DNA
- Plasmids
- extra DNA in cytoplasm
- Round shape
- replicate autonomously
- NOT vital Genes
- Accessory genes like pili
or antibiotic resistance
Lec 4
- Cytoplasmic DNA
- Plasmids
- Transference
- Plasmids can be
transferred from one
microbe to another
- this is used to create dif
strains w/ different
attrib.
Lec 4
- Prokaryotic Cytoplasm
- Lots of ribosomes
- very viscous (4x eukary)
- less viscous if starving
- many vesicles and inclusion
- some make endospores
Lec 4
- Gas vesicles
(cyanobacteria)
- in both Bacteria & Archea
- found in photosynthetic org
- used to confer buoyancy
- change depth for optimal
photosynthesis
Lec 4
- Gas vesicles
- structure
- outside made of GvpA & GvpC
- GvpA is rigid Beta sheet
and impermeable H20/gas
- GvpC is alpha helical and
cross linked (make linkage)
Lec 4
- Cell inclusions
- Storage Granules
- Hold food stuffs for cell
- is non unit (single layer)
- not bilayer
Lec 4
- Cell inclusions
- Storage Granules
- PHA
PHA = polyhydroxyalk
Lec 4
- Cell inclusions
- Storage Granules
- PHB
- most common storage for excees carbon
- most common storage period..
Lec 4
- Cell inclusions
- Storage Granules
- glycogen inclusions
- glycogen inclusions
- some cells use glycogen for e- metabolism
Lec 4
- Cell inclusions
- Storage Granules
- Magnetosomes
- store Fe3)4, magnetite
- allows microbe to orient
along magnetic field
- non unit (unilayer)
Lec 4
- Cell inclusions
- Storage Granules
- Sulfur
- microbes that oxidize H2S
- stores elemental Sulfur
Lec 4
- Cell inclusions
- Storage Granules
- Phosphate
- phosphate stored as
polyphosphate in granules
Lec 4
- Cell inclusions
- Storage Granules
- inclusion bodies
- store any excess protein
lying around
- catch all name
Lec 4
- Other 'somes
- Carboxysomes
- any microbe that fixes
CO2
- contain protein RuBisCo
- CO is Carboxylate
Lec 4
- Other 'somes
- Enterosomes
- Found in the intestine
- contain proteins to convert
propanediol for metabolism
- ex is salmonila
Lec 4
- Endospores
- Survival mechanism
- only made when microbe is
under stress (suicide)
- called sporulation
- 3 classifications
Lec 4
- Endospores
- terminal endospore
- endospore forms at far end
Lec 4
- Endospores
- Subterminal
- endospore forms close to a
far end
Lec 4
- Endospores
- central
- endospore forms close to center of microbe
Lec 4
- Endospores
- longevity
- can survive dessication,
heat, and temp
- can last for centuries
Lec 4
- Endospores
- Diversity
- Microbes that form endospor
are all related
- most (all) are gram +
- either bascillus or clostridium
-