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10 Cards in this Set
- Front
- Back
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Components of a Microscope
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2. Stage
2. Illumination 3. Abbe Condenser 4. Body Tube 5. |
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Theoretical Principles of Microscopy
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1. Magnification
2. Resolving Power / Resolution 3. Resolving power = wavelength of light/2 (numerical aperture) 4. Illumination 5. Working Distance |
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Steps to get to 100X
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1. Use the 10X objective first; resolve
2. Use the 45x objective; resolve 3. Put in oil immersion 4. Swing 100x into place |
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Stage
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-A fixed platform with an opening in the center.
-In addition to the fixed stage, most microscopes have a mechanical stage - |
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Illumination
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-Light source is in the base of the instrument
-Some microscopes have a built-in light source -If microscope has a mirror, an external light source must be used |
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Abbe Condenser
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-Found under the stage
-Has two lenses that collect and concentrate light -Iris Diaphragm regulates amount of light entering the lens system |
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Body Tube
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-Upper end has ocular lens
-Lower end has moveable nosepiece that contains the objective lens |
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Magnification
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Total magnification obtained by multiplying the ocular magnification by the objective magnification
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Resolving Power
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Wavelength of light / 2
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Refractive Index
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-Relationship of resolving power to wavelength only works when light is exactly parallel.
-Bending of light going from air to the glass means that less light = less resolution -Immersion oil compensates for this by replacing air in the space between the specimen and the lens. Immersion oil has the same refractive index as glass. |
