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25 Cards in this Set

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Koh or PA stain shows MULTIPLE BRANCHED spetate dark pigmented hyphae and elongated budding cells?

what must these lesions be dddx from?
tinea nigra


ddx from malignant melanoma or contact dermatitis
KOH or PAS stain reveals hair shafts containing conidia w/ white nodules and HYALINE, SPETATE HYPHAE broken up into ARTHROSPORES
white piedra
KOH prep shows fragments of mycelium w/ or w/o branches and septa

what may be seen?
Otomycosis


may see condiophores and conidia or sporangiosphores
KOH and PAS show hyphae with microconidia
*Tinea faciei
*Tinea barbae
KOH and PAS show chains of macroconidia and hyphae on HAIR SHAFT
*Tinea capitis ectothrix
Conidia and Hyphae seen INSIDE hair shafts on KOH or PAS
TInea capitis endothrix
KOH and PAS show mycelia and conidiospores

*club shpaed macrconidia w/ rough walls
Tinea corporis


club shaped microconidia from microsporum canis
hyphae an dmacroconidia seen on PAS or KOH or nail specimen
tinea unguium
skin scrapings show masses of hyphae and macroconidia
Tinea manumm
PAS and KOH reveal hyphae and cnoidia

some species will exhibit club shaped macroconidia

what will not be prodduced
TInea cruris

epdermophytona and trichophtyon have club shaped

epidermophyton foccosum does NOT have microconidia
taken from in b/w toes or fingers the PAS and KOH show hyphae and conidia?
tinea pedis
KOH and PAS show granules of BROAD, BRANCHING SEPTATE HYPHSE with dense NEUTROPHILIC Exudates occuring as BLACK GRAINS
Pseudallescheriasis
PAS show chlamydospores in ASTEROID BODeS and cigar shaped budding yesat forms in pus filled granulomas
sporotrichosis
exudates of infected tissues or blood have chains or OVAL to GLOBOSE budding yeast cells or pseudohyphae with constrictions at septa dn w/ oval blstospores near septa
Candidiasis
PAS stain shows individual spherical to elongate budding, nonencaspulated yeast like cells
Rhodotorulosis
Mehtenamin silver nitrate staining os sputum from bonchoalveolar lavage or a lung bx reveals THICK WALLED CRESCENT SHAPED CYSTS with many INTERNAL BODIES

what is another possible stain test?

can this yeast be cultured?
Pneumocystosis


can also wright stain and direct fluorescent stain

CANNOT be cultured
nigrosin staining of cerebral spinal fluid or exudate show GLOBOSE yeast cells with THICK polysaccharide capsules fomring hALOS around the cells
Cryptococcosis
conidial structures or "ACUTE ANGLE" branching, septate hyphae in PAS or KOH

what other preps can be tested?
Aspergillosis


preps of sputum or bronchoalveolar lavage
PAS prep of tissue exudates shows septate hyphae with oval or spherical arthrospores
Geotrichosis
various forms of hyphal elements(branching or non branching hyphae or pseudohyphae) are found on KOH or PAS scrapings of skin or nails
Onychomycosis
KOH or PAS shows broad, branching non septate or sparsley setpate hyphae with right angle branching
Mucormycosis
KOH, calcofluor white, methenamine silver or PAS stain of tissue prep shows LARGE, BROAD based budding yeast cells

mold forms gorw at romm tem and yeasts grow at 37 degrees C
BLastomycosis
KOH or PAS stain of tissue demonstartes thin walled SPHERULES with endospores
Coccidioiodmycosis
exudate cells contain extra or intracellular oval yeasts with prominent bud scars and thin walls

fungus is thermally dimorphic
histoplasmosis
what indicates an unfavorable dx?
1. increased complement fixing or precipitating antibody titers