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13 Cards in this Set
- Front
- Back
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How does a simple microscope differ from a compound microscope?
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simple has basic lense, erly day microscopes
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Define: magnification, resolution(resolving power), and refraction. What determines the resolving power of a light microscope?
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magnification- size
resolution- clarity between objects resolution power determined by wavelengths |
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For what special application might a dark field microscope be used?
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looking at living cells
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Why must specimens be stained for brightfield microscopy?
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bacteria are colorless
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How do electron microscopes differ from light microscopes in regards to focusing and illumination?
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electron microscopes use electron beams instead of light waves
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Name two types of electron microscopes and how their applications differ.
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TEM- view detailed structures of cells and viruses
SEM- 3d image of specimen |
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What type of microscope is needed to study viruses?
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electron microscope
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In the negative staining technique, the cell or cell part does not absorb the dye but remains colorless. Is that a true statement?
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yes
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For what is the hanging drop procedure used?
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to view cell motility
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The most important stain technique in microbiology is? Describe the mechanism of this technique.
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Gram stain
crystal violet->gram's iodine->alcohol->safranin |
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The bacterial cell has what kind of electrical charge on its surface?
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negative
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Why do acid-fast organisms need a different kind of staining procedure?
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mycolic acid in cell walls prevents penetration by grams stain
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How do differential stains differ from simple stains?
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simple- single dye
differential- multiple dye |
