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15 Cards in this Set
- Front
- Back
- 3rd side (hint)
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In the process of induction is the repressor usually bound to the operator?
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yes
example of induction? |
Lac operon only being expressed when lactose is present
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In the process of repression is the repressor usually bound to the operator?
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no
example of repression? |
trp operon. halts production of tryptophan if it is present
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what is special about the 16SRNA segment of bacterial genomes?
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gene is unique for each species of bacteria, but is flanked by conserved sequences. Allows for identification of Bac by PCR seq.
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A strain of salmonella typhimurium is described as having the phenotype his+ .
In other words it... |
Can grow on medium that lacks the amino acid histidine
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A strain of salmonella typhimurium is described as having the phenotype lac+ .
In other words it... |
Is able to survive on an agar plate provided with lactose for nutrition. (it can digest lactose)
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A strain of salmonella typhimurium is described as having the phenotype cat+ .
In other words it... |
Is resistant to Chloramphenicol.
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An infection by which bacterium can not be treated with kanamycin?
S. aureus kan S. epidermidis amp E. coli kan- S. typhimurium cat |
S. aureus kan
(Kan implies that it has resistance to Kanamycin) |
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What is an R factor?
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non-chromosomal DNA with antibiotic resistance
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can bacterial mutations cause antibiotic resistance?
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yes but this is very rare.
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Name the process:
Transfer of DNA from a bacterium to another by a lytic phage |
generalized transduction
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Name the process:
Transfer of DNA from a dead bacteria to a live one |
transformation
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Name the process:
Transfer of DNA from a Hfr Bac to a F- Bac |
conjugation
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Name the process:
Transfer of DNA from a bacterium to another by a lysogenic phage |
specialized transduction
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differences between an F factor and a lytic phage
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Both can be either integrated or not.
Both can carry bac DNA from the integration site F factors carry antibiotic-resistant genes Lytic phages carry toxins F factors do not kill the host bacteria |
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Why is gene exchange between bacteria rare?
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modifying enzymes (methyl gps)
restriction enzymes (digest DNA) |
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