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27 Cards in this Set
- Front
- Back
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Taxonomy
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categorization of organisms into order. Helps show evolution
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Binomial system
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Genus species
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Taxonomy rank
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DDCOFGSS -Domain, Division, Class, Order, Familly, Genus, Species, Strain
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Who is responsible for the 5 kingdom system?
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Whittaker
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Woese theory
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Archea are ancient Lack muramic acid in cell wall,
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Numerical Taxonomy
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use enterotube and results by number and find org by number on computer (doesn't always work especially when bac is changed)
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G + C taxnomy
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melting temperature
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Nucleic acid hybridization
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read and compare DNA sequences
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DNA probes
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can be used from identification
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rRNA and phylogeny
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shows relationships especially between 16s rRNA
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Isolation of rRNA
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lysis, phenol extraction, RNA ppt with alcohol and salt, PCR to amplify
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Biggest micro manual
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Bergey's
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Stages of syphilis
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1. 10-90 chaucre like a zit on the face 2. septicema, communicable
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shirochetes features
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gram neg, slender coils. periplasmic flagella (2-100). Cytoplasm and nuclear region are in cell membrane-cell wall complex (protoplasmic cylinder)
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Shirochetes 5 genera
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Bored Christ tripped spiraling leper. Borrelia, Christispira, treponema, spirochaeta, leptospira
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Treponema pallidum
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syphillis
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Treponema pertenue
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yaws - a tropical disease on soles of feet and palms
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treponema carateum
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tropical disease that causes hyperpigmentation
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Borrelia burgdorferi
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causes lyme disease
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Leptospira interrogans
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leptospirosis - flu symptoms and liver disease
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Some ____ live symbiotically in insects and help them digest cellulose
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shirochetes
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Genus Spirillum
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largest bac. Freshwater and marine. recycle matter
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C. jejuni
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gastroenteritis
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Bdellovibrio
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Gram - should not get into bed with these. small bac that parasitize gram neg. live in periplasmic space
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What are restriction endonucleases?
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recognize and cleave certain sequences in DNA. 500 kinds. Leaves sticky overhangs
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Steps of Gene cloning (4)
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1. isolate or synthesize gene of interest. 2. incorporate it into the a cloning vector. 3. vector into host. 4. detection of gene in host
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Plasmids as cloning vectors
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Small (1,000 to 300,000 bps). can replicate independently of bacteria genome. multiple in cell means it can be amplifiied.
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