Micro Test 2

Front 1

Associated with infections of every area of the body especially: Urinary Tract, Gi Tract, Wounds, Blood

Back 1

enterics

Front 2

Glucose fermenters and use glucose anerobically

Back 2

Enterobacteriacea

Front 3

Facultative anerobes that reduce nitrate to nitrites

Back 3

Enterobacteriacea

Front 4

Most Enterobacteriacea are motile at body temp. 37*C. The exceptions that are not motile are:

Back 4

Shigella
Klebsiella
Yersinia

Front 5

associated with the cell wall, heat stable, and ENdotoxin production (E.coli o157)

Back 5

O or somatic antigen

Front 6

Surrounds cell wall, Heat LABILE
Klebsiella, Salmonella, and E. Coli posses this antigen

Back 6

K or envelope antigen

Front 7

Found only in the MOTILE enterics: heat Labile and used to serotype Salmonella species

Back 7

H or flagella antigen

Front 8

Grows on BAP in waves
"swarming"

Back 8

Proteus

Front 9

Pink colonies on McConkey plate indicate lactose fermenters:

Back 9

E.coli, Klebsiella, Enterobacter

Front 10

Associated with infections of every area of the body especially: Urinary Tract, Gi Tract, Wounds, Blood

Back 10

enterobacteriaecea

Front 11

I
m
v
c

Back 11

indole
methyl red
voges-proskauer
citrate

Front 12

Glucose fermenters and use glucose anerobically

Back 12

Enterobacteriacea

Front 13

Facultative anerobes that reduce nitrate to nitrites

Back 13

Enterobacteriacea

Front 14

Most Enterobacteriacea are motile at body temp. 37*C. The exceptions that are not motile are:

Back 14

Shigella
Klebsiella
Yersinia

Front 15

associated with the cell wall, heat stable, and ENdotoxin production (E.coli o157)

Back 15

O or somatic antigen

Front 16

Surrounds cell wall, Heat LABILE
Klebsiella, Salmonella, and E. Coli posses this antigen

Back 16

K or envelope antigen

Front 17

Found only in the MOTILE enterics: heat Labile and used to serotype Salmonella species

Back 17

H or flagella antigen

Front 18

Grows on BAP in waves
"swarming"

Back 18

Proteus

Front 19

Pink colonies on McConkey plate indicate lactose fermenters:

Back 19

E.coli, Klebsiella, Enterobacter

Front 20

I
m
v
c

Back 20

indole
methyl red
voges-proskauer
citrate

Front 21

Removal of an amino group from an amino acid

Back 21

deaminase reaction

Front 22

removal of a carboxyl group from an amino acid

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decarboxylation reaction

Front 23

detects low lactase fermenter

Back 23

ONPG

Front 24

Determines if an organism possesses the enzyme TRYPTOPHANOSE

Back 24

Indole

Front 25

determines if an organism is capable of producing and maintaining an ACID environment.

Back 25

Methyl Red

Front 26

Acids are intermediate metabolites in:

Back 26

pyruvate metabolism

Front 27

Detects ACETOIN.

Back 27

Vogues-Proskauer

Front 28

Acetoin is an intermediate metabolite in

Back 28

pyruvate metabolism

Front 29

Determines if an organism can use citrate as is ONLY source of Carbon

Back 29

Citrate

Front 30

Provides the initial step in the identification of Enterobacter

Back 30

Triple sugar Iron slant
(TSI slant)

Front 31

Ferrous state in TSI slant is used to detect:

Back 31

H2S production

Front 32

Indicator in TSI is

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phenol red

Front 33

Slant portion of TSI is ____ and the deep portion or "butt" is _____

Back 33

aerobic, anerobic

Front 34

TSI media will turn yellow based on what?

Back 34

Carbohydrate utilization

Front 35

K/A: alkaline/acid

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Red slant/Yellow Butt
utilized glucose only

Front 36

A/A

Back 36

Both slant and butt are acid (yellow)
Fermented 2 to 3 sugars

Front 37

K/K alkaine/alkaline

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Both slant and butt are red (alkaline)
non fermenter

Front 38

Enterohemorrhagic E. Coli. o157:H7 is

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sorbitol NEG

Front 39

Pink colonies on MacConkey, Green Sheen on EMB, ONPG pos, IMVC ++--

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E.coli

Front 40

Humans are natural reservoirs for

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Shigella

Front 41

Enterotoxin invades large bowel causing fever, cramps, watery diarrhea the turns into a bloody mucoid diarrhea. Neurotoxin can cause paralysis and death

Back 41

S. Dysentery

Front 42

"do nothing" organism

Back 42

Shigella

Front 43

Clear colonies on MacConkey
Methyl red pos
d-mannose pos

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Shigella

Front 44

Klebsielleae Tribe

Back 44

Klebsiella
Enterobacter
Serratia

Front 45

Produce large amounts of gas in TSI deep
Pink mucoid colonies on MacConkey

Back 45

Klebsiella

Front 46

Two important species of Klebsiella

Back 46

K. pneumoniae
K. oxytoca

Front 47

K. Pneumoniae is indole ___
K. oxytoca is indole _____

Back 47

negative, positive

Front 48

Accounts for 90% of all Klebseilla isolates. resistant to AMPICILLIN and causes neonatal Meningitis

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k pneumo

Front 49

IMVC --++

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K. pneumo

Front 50

Causes septicemia, UTI, and wound infections
IMVC +-++
Pink mucoid on Mac

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K oxytoca

Front 51

Ornithine decarboxylase is Positive

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Eneterobacter

Front 52

Found in soil, water, Dairy products

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Enterobacter

Front 53

Arginine dihydrase positive ADH

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e. Cloacae

Front 54

Lysine decaboxylase LDC Positice

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E. aerogenes

Front 55

opportunistic pathogen commonly seen in nosocomial infections. Can be present on hands of healthcare workers

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Serratia

Front 56

Most important species of Serratia. Muti drug Resistant.

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S. Marcescens

Front 57

Blood red colonies on Mac

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S. Marcescens

Front 58

Ornithine decarboxylase is Positive

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Eneterobacter

Front 59

Salmonella species typically isolated from humans and warm-blooded animals

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Salmonella enterica. (always considered pathogen)

Front 60

Found in soil, water, Dairy products

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Enterobacter

Front 61

classification based on O and H antigens

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Kauffman-White

Front 62

Arginine dihydrase positive ADH

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e. Cloacae

Front 63

Most common illness in salmonella that occurs from food poisoning

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Gastroenteritis

Front 64

Lysine decaboxylase LDC Positice

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E. aerogenes

Front 65

opportunistic pathogen commonly seen in nosocomial infections. Can be present on hands of healthcare workers

Back 65

Serratia

Front 66

Most important species of Serratia. Muti drug Resistant.

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S. Marcescens

Front 67

Blood red colonies on Mac

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S. Marcescens

Front 68

Salmonella species typically isolated from humans and warm-blooded animals

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Salmonella enterica. (always considered pathogen)

Front 69

classification based on O and H antigens

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Kauffman-White

Front 70

Most common illness in salmonella that occurs from food poisoning

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Gastroenteritis

Front 71

Salmonella serotype typhi causes serious sepsis known as

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Typhoid fever

Front 72

Clear green colonies with black centers on Hektoen media

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Salmonella

Front 73

Clear green colonies are lactose ___

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Negative

Front 74

Proteeae tribe consists of

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Proteus
Providencia
Morganella

Front 75

Lactose Neg
Deaminase Pos
Methyl Red Pos

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Proteeae

Front 76

Swarming motility
UREASE rapidly pos
h2s pos

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Proteus

Front 77

Proteus that is indole Neg and ODC pos

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p. mirablis

Front 78

Proteus that is indole pos and ODC neg

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p. vulgaris

Front 79

Usually associate with nocomial infections of the urinary tract and skin
MANNOSE POS

Back 79

Providencia

Front 80

Resemble salmonella but are ONGP pos and LDC neg

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Cirtobacter

Front 81

Associated with GI infections in the tropics and subtropics

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Edwardsiella

Front 82

Small, clear pinpoint colonies on Mac and non-motile at 37*

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Yersinia

Front 83

Agent in bubonic plague
fleas spread infection

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y. Pestis

Front 84

causes entercolitis especially in children and infants. PSEUDO APPENDICITIS if mesenteric lymph nodes become enlarged

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y. Enterocolitica

Front 85

Incubation period is no more than 7 days after the bite of an infected flea. 75% death rate

Back 85

Bubonic Plague

Front 86

Incuation period is -2-3 days. Death rate in untreated is >90%

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Pneumonic plague

Front 87

usually occurs with bubonic plage as an acute, fulminant illness

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septicemic plague

Front 88

agents of bioterrorism

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E.coli o157
Salmonella
Yersinia Pestis

Front 89

Natural inhabitant of Sea water

Back 89

Vibrio

Front 90

All species of Vibrio require an increased salt concentration for in Vitro growth. (halophilic) EXCEPT

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v. cholera and v mimicus

Front 91

facultative anerobe with prefrence for increased CO2. All ferment glucose

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Vibrio

Front 92

potent cholera enterotoxin

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choleragen

Front 93

"rice water" stools

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Cholera

Front 94

Exibits DARTING or Shooting Star motility.
Can be divided into serogroups based on O antigen

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V. cholera

Front 95

Epidemics of intestinal cholera are most often caused by

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V. cholerae O1

Front 96

extraintestinal infections of V. cholerae are often caused by

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non-O1 V. cholera

Front 97

Most commonly isolated Vibrio species

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V. Parahaemolyticus

Front 98

Infection usually occurs after ingestion of contaminated shellfish

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V. Parahaemolyticus

Front 99

Infection typically occurs after exposure to seawater

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Vibrio alginolyticus

Front 100

Infection typicially occurs after ingestion of raw oysters. High mortality rate

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V. vulnificus

Front 101

Disease is known to occur mainly in individuals with pre existing liver disease. Likely due to increase availabilty of iron

Back 101

V. vulnificus

Front 102

Virbrio that is not cultured immediately should be transported in what medium?

Back 102

Cary-Blair Transport

Front 103

Selective and differential for isolating Vibrio species

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TCBS agar.

Front 104

Natural inhabitant of Sea water

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Vibrio

Front 105

All species of Vibrio require an increased salt concentration for in Vitro growth. (halophilic) EXCEPT

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v. cholera and v mimicus

Front 106

facultative anerobe with prefrence for increased CO2. All ferment glucose

Back 106

Vibrio

Front 107

potent cholera enterotoxin

Back 107

choleragen

Front 108

"rice water" stools

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Cholera

Front 109

Sucrose fermenters that produce yellow colonies on TCBS

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V cholera and alginolyticus

Front 110

Sucrose non fermenters that produce blue green colonies on TCBS

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V. vulnificus and parahaemolyticus

Front 111

intestinal infections usualy occur in patients who have history of eating seafood or drinking untreated well water

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Aeromonas

Front 112

Aeromonas that is lactose positive, cause it to resemble e.coli or enterobacter

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A. Caviae

Front 113

Aeromonas is oxidase ___

Back 113

positive

Front 114

Enterics are oxidase ____

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negative

Front 115

Produces bulls eye colonies on CIN agar. May be isolated from fecal specimen with blood agar containing ampicllin

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Aeromonas

Front 116

Only species in plesiomonas genus

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shigelloides

Front 117

selective agar fro P. shigelloides because colonies appear white to pink

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Inositol brilliant green bile salts. IBB

Front 118

A nonfermenter should be expected if GNR is

Back 118

oxidase pos

Front 119

all members of ___ are oxidase Neg

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Enterobacter

Front 120

TSI is not suitable to study the carbohydrate use of ______

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nonfermenters

Front 121

___ medium is used to study carbohydrate use by nonfermenters

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OF

Front 122

All member of ___ will ferment glucose anaerobically and aerobically

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Enterobacter

Front 123

Contains high concentration of carbs and low concentration of peptones

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OF medium

Front 124

Indicator of the OF medium

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Bromothymol blue.
Pos is yellow
neg is blue green

Front 125

Organisms can only use the carbs when oxygen is present

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oxidative

Front 126

can metabolize glucose in the presence or absence of oxygen

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Fermentative

Front 127

Cannot utilize carbs/sugars as an energy source

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Asaccharyolytic

Front 128

The most frequently isolated non fermenter

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Pseudomonas aeruginosa

Front 129

Strict aerobe and "water lover" Causitive agent of swimmers ear

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P. Aeruginosa

Front 130

Strict aerobe, GRAPE LIKE SMELL, Metallic sheen, 35 and 42*c

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P. Aeruginosa

Front 131

Often isolated from the respiratory tract of patients with cystic fibrosis

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P. aeruginosa

Front 132

P. aeruginosa is the only organism that produces what kind of pigment

Back 132

Pyocyanin

Front 133

Oxidase neg, DNase POS
AMMONIA odor
LAVENDER-GREEN pigment on bap

Back 133

S. Maltophilia

Front 134

Causes Cepacia syndrome in patients with CF

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Burkholderia

Front 135

EARTHY odor,Lysine decarboxylase pos,
Non-fluorescent yellow pigment

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Burholderia cepacia

Front 136

GNR resist decolorization

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Acinetobacter

Front 137

Beta-hemolytic/non-hemolytic

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Acinetobacter

Front 138

Plump GN coccobacilli

Back 138

Moraxella

Front 139

May pit agar surface on BAP and is asaccharolytic

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Moraxella

Front 140

Pleomorphic GNR with faint staining

Back 140

Legionella

Front 141

Serogroup of legionella responible for >90% of human infections

Back 141

Pneumonophilia

Front 142

Aerobic organism that grow best under 5% CO2

Back 142

Legionella

Front 143

Media is a cysteine-enriched media that should be used when legionella is suspected

Back 143

Buffered charcoal yeast extract. BCYE

Front 144

Will not grow on ordinary micromedia. Hippurate pos

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L. Pneumo

Front 145

GN diplococci. Kidney bean/coffee bean shaped

Back 145

Neisseria

Front 146

Almost all Neisseria species are ___ pos.

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catalase

Front 147

All Neisseria species are ___ and ___ pos

Back 147

Oxidase and DNase

Front 148

Hair like structures that extend from the cytoplasmic membrane through the outer membrane

Back 148

Pili

Front 149

Gonococcus/GC
May cause conjunctivitis in adults and newborns

Back 149

Neisseria Gonorrhoeae

Front 150

Skin lesions, septicemia, endocarditis, gonococcal arthritis

Back 150

DGI.

Front 151

Gonococcal ___ are associated with virulence and affect antibody formation, leukocyte response, and phagocytic killing

Back 151

outer membrane proteins

Front 152

Recommended medium for GC cultures

Back 152

MTM.

Front 153

GC is positive for glucose and negative for maltose, lactose and sucrose on what agar?

Back 153

CTA

Front 154

Inhibits GPC

Back 154

vancomycin

Front 155

inhibits GNR

Back 155

coliston

Front 156

Inhibits yeast

Back 156

Nystatin

Front 157

Inhibits swarming proteus

Back 157

trimethoprim

Front 158

Most common direct method of identification for N. gonorrhoea

Back 158

Nucleic acid probe

Front 159

All GC should be test for

Back 159

beta-lactamse

Front 160

chemically bonded ring in the penicillin molecule

Back 160

beta-lactamase

Front 161

can be carried in the nasopharynx in asymptomatic individulas

Back 161

Menigitidis

Front 162

Organism in encapsulated Resists phagocytosis

Back 162

N. Meningitidis

Front 163

causes death in <24 hours due to ENDOTOXIN release. (TNF)

Back 163

Acute Meningococcemia

Front 164

Unlike N. Gonorrhoea,____ will grow on BAP

Back 164

N. Meningitidis

Front 165

Only Neisseria that is lactose pos

Back 165

N. Lactimica

Front 166

Respiratory pathogen formally called Branhamella Catarrhalis

Back 166

Moraxella Cat.

Front 167

Commonly produces beta-lactamase, is DNase pos and reduces nitrates

Back 167

M Catarrhalis

Front 168

"blood lover" Reduces nitrates and facultative anerobe

Back 168

Haemophilus

Front 169

Requires Choc agar because it contain Hemin. (xfactor)

Back 169

Haemophilus

Front 170

BAP is NOT adequate to isolate _____ because sheep RBC release DNase

Back 170

Heamophilus

Front 171

Synthesizes NAD (v-factor)

Back 171

s. aureus

Front 172

aka pfeiffers bacillus. 6 serotypes/8biotypes

Back 172

h. influenza

Front 173

Encapsulated strains are pathogenic, especially in children <3yrs

Back 173

h. Influenza

Front 174

all h. influenza isolates should be tested for

Back 174

beta-lactamase

Front 175

Cause of pink eye

Back 175

H. aegypticus

Front 176

VD characterized by chancroid
"school of fish"

Back 176

h. Ducreyi