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10 Cards in this Set
- Front
- Back
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These are traditional methods of diagnosis of ID
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Culture. Staining. Serology.
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These are molecular methods of diagnosis of ID
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Amplification: PCR, TMA, LCR, bDNA. Hybridization (outdated and insensitive). Strain comparison: Electrophoresis, Restriction Fragment analysis.
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Sensitivity (test)
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Ability of test to correctly identify positives.
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Specificity (test)
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Ability of test to correctly identify negatives.
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TMA
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Transcription Mediated Amplification
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LCR
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Ligase Chain Reaction
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bDNA
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Branched chain DNA Amplification
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Quantitative amplification
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Used to determine viral load--amplify an unknown amount of viral proteins for a known amount of time to determine the initial amount of viral protein.
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Qualitative amplification
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Used to detect even a miniscule amount of microbe protein--only useful when detection of any evidence at all correlates with disease
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These organisms are detected by molecular techniques
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VIRUSES--herpes, enteroviruses, respiratory, HIV, hepatitis. BACTERIA--pertussis, gonococcus, C. trachomatis, Group A&B Strep, Staph aureus, TB, Mycoplasma pneumoniae, Borelia burgdorferi, etc. FUNGI, PROTOZOA.
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