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10 Cards in this Set
- Front
- Back
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What organism does the P1 phage infect?
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E. coli
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What orgainsim does the P22 phage infect?
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Samonella
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How can individual phages be purified?
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Based on their ability to lyse cells and form plaques in a bacterial lawn.
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What are the 5 steps a virus's life cycle?
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1. Attachment,
2. Penetration, 3. Synthesis of nucleic acid and protein, 4. Assembly and packaging, 5. Release (lysis) |
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Where does packing of P22 phageheads initiate?
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At a pac site
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"Headful Mechanism"
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The mechanism in which phages are packaged, where the amount of DNA packaged is somewhat higher than the phage genome. Subsequent phageheads initiate packing where the previous phagehead finishes.
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How is Temperate bacteriophage lambda different from P22?
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Lambda has two different lifestyles, it can enter a lysogenic or lytic pathway.
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In what type of pathway would viral DNA be integrated into host DNA?
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Lysogenic pathway
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Lysogen
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The cell containing the prophage.
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Where do genes need to be located in order to be taken up by the phage?
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Near the attachment site.
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