Micro Lab Test 2

Front 1

First bacterium shown to be the cause of a disease? (Robert Koch)

Back 1

Bacillus Anthracis (Anthrax)

Front 2

The thick protein coat make up of keratin surrounding an endospore is called?

Back 2

Exosproium

Front 3

What is an Endospore?

Back 3

1.) Dormant form of bacteria.
2.) Produced when cells are stressed.
3.) Resistant to: Bacillus and Clostridium.

Front 4

What does Acid Fast mean?

Back 4

Resistant to decolorizing by adding alcohol after staining.

Front 5

A smooth glycocalyx is referred to as a?

Back 5

Capsule

Front 6

What are Flagella?

Back 6

Thin structures used for motility.

Front 7

Name the Flagella Arrangements:

Back 7

1. A single flagellum can extend from one end of the cell is said to be Monotrichous.

2. A single flagellum can extend from both ends of the cell - Amphitrichous.

3. Several flagella (tuft) can extend from one end or both ends of the cell - Lophotrichous

4. Multiple flagella may be randomly distributed over the entire bacterial cell - Peritrichous.

Front 8

What is the purpose of the Quadrant Streak Plate Method?

Back 8

To isolate single colonies. (Single colonies arise from a single cell)

Front 9

pH Range

Acidophiles:

Neutrophiles:

Alkaliphiles:

Back 9

Below pH5.5

Between 5.5 and 8.5

Above pH8.5

Front 10

What happens when you place an organism outside its normal pH range?

Back 10

You denature the proteins and disrupt the membranes.

Front 11

What kind of stain is used for Flagella and why?

Back 11

Mordant Stain is used to enhance or make larger.

Front 12

What are Capsules made up of and what do they do?

Back 12

Polysaccharides and polypeptides and they adhere and evade phagocytosis.

Front 13

What are the Acid Fast Pathogens?


Acid Fast Genus?

Back 13

Tuberculosis and Leprosy


Myco-bacterium

Front 14

Ubiquity: Open Plate Tests for what?

Back 14

Air contamination

Front 15

Ubiquity: Closed Plates test for what?

Back 15

Media Sterility

Front 16

3 hand washing micro-biota

Back 16

1.) Diptheriod: Gram + bacteria ie: Diptheria

2.) Staphylococci: Non-pathogenic Staphylococci Epidermidis (SE) and potentially pathogenic Staphylococci Aureus (SA)

3.) Yeasts and Fungi: Normal inhabitants of the skin

Front 17

Basic Aseptic Technique

Back 17

1. Clean workspace w/ vespene
2. Wash hands
3. Work near open flame
4. Always heat flame inoculation tools
5. "Briefly" heat flame open culture tube

Front 18

Quad Streak Method

Back 18

1. Heat flame loop and top of open tube.
2. Touch loop to bacteria (2 sec).
3. Streak bacteria on plate in "quad" formation.
4. Incubate @ 25 degree C. label side up.