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33 Cards in this Set
- Front
- Back
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Binary Fission
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the replication of bacteria; asexual reproduction
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generation time
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the time it takes for the number of bacteria to double
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Lag Phase
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a period of adjustment for the bacteria after they are introduced into the new medium
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Log phase
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doubling time most accurate here; growth rate maximum; will stay in this phase until nutrients run out
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stationary phase
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declining growth rate; can remain in for a long period of time
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Absorbance measures ____ cells.
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live and dead
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Titer measures _____ cells.
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only live
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Bateriostatic
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cell number levels off and stays constant, turbidity levels off and stays constant
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Bactericidal but non-bacteriolytic
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cell number drops dramatically, turbidity levels off and stays constant
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Bactericidal and bacteriolytic
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cell number drops dramatically, turbidity drops dramatically
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Give details about Vibrio natriegens.
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- salt concentration is important
-short generation time (10-11 min.) -we could see all three phases during class |
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Prototrophs
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wild-type organisms that can synthesize all the compounds required for growth
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What does fastidious mean?
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have more complex nutritional requirements
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Why is blood agar used sometimes?
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to observe the differential hemolytic activity of streptococci
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What are proteins that kill animal cells by disrupting their membranes?
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Hemolysins
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Alpha hemolysis
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partial breakdown
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Beta hemolysis
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complete breakdown
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Gamma
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no hemolysis
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CNA agar is selective for?
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Gram positive bacteria
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What drugs are the selective components of CNA?
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Colistin and nalidixic acid
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What are enteric bacteria?
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a group of closely related bacteria that can be found in the intestines of humans
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Give some characteristics of enteric bacteria.
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-gram negative rods
-straight or slightly bent -faculative anaerobes -non-spore forming -glucose fermenters -catalase positive |
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What compound makes the CNA plate differential?
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RBC
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KIA agar contains ___% glucose and ____% lactose.
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0.1/1.0
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What are the differentiation indicators in the KIA medium?
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ferrous sulfate and sodium thiosulfate
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What reagent is used in the phenylalanine deaminase test? What will a positive reaction look like?
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ferric chloride (FeCl3) / a green color
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What does the citrate test determine?
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the ability of an organism to use citrate as its sole source for carbon growth
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What is the carbon source in the citrate test?
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sodium citrate
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The use of salts of organic acids leads to the formation of ____ carbonates? (like in the citrate test)
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alkaline
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What is the indicator in the citrate test?
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Brom thymol blue
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What dye is used to stain DNA?
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ethidium bromide
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What is serology?
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the study of antigen-antibody interactions in vitro
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What is agglutination?
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the visible clumping of a particulate antigen by specific divalent antibodies that form bridges between antigenic determinants on adjacent cells
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