Micro Lab Quiz

Front 1

A properly prepared smear

Back 1

Withstands the washings required during the staining procedure (i.e. the bacteria doesn’t wash off)

Is not too thick (this can inhibit proper staining throughout)

Does not have excessive heat fixing (this can cause distortion and shrinkage of the cells)

Front 2

Heatfix

Back 2

passing the slide sample side up through the flame 3-4 times.

Front 3

why heat fix

Back 3

kill organism so its not potential pathogen

keeps bacteria from being washed off with oil

exposes stainable properties of cell by denaturing proteins

Front 4

coccus

Back 4

round

Front 5

bacillus

Back 5

rod shape

Front 6

spiral

Back 6

twists

Front 7

diplo

Back 7

pairs

Front 8

strepto

Back 8

chain like

Front 9

staphylo

Back 9

cluster

Front 10

spirillum

Back 10

rigid spirals

Front 11

spirochete

Back 11

flexible spirals

Front 12

simple stain

Back 12

color microbe to examine shape/ arrangement; 1 dye only

Front 13

basic dye

Back 13

it has a positive charge; are attracted to negative charge created by the phospholipids in the membranes.

Front 14

methylene blue

Back 14

used for slides with yeast

Front 15

crystal violet

Back 15

for most slides.

Front 16

common dyes in simple stains

Back 16

crystal violet

methylene blue

safranin

Front 17

Differential stain

Back 17

•Stain technique that differentiates between 2 different types of cellular structures.

Front 18

differntial stain is used to determine

Back 18

–Size

–Morphology

–Arrangement

Front 19

Gram +

Back 19

blueish purple

Front 20

Gram -

Back 20

pinkish red

Front 21

gram stain technique

Back 21

1)Next add Primary (1°) stain = first stain applied:CrystalViolet (1 min)

3)Wash slide with distilled water

4)Next add Mordant: chemical that increases size and interaction of CV with peptidoglycan:Iodine (1 min)

5)Wash slide with distilled water●

6)Add Decolorizing agent (acetone alcohol)2-4 drops running down slide over 2 secs:(removes1° stain Crystal Violet from Gram (-) cells)●

7)Wash slide with distilled water

8)Add Counterstain:Safranin (2min)(The second stain is applied to stain decolorized cells)

Wash with distilled water, blot, and observe via oil immersion

Front 22

endospore

Back 22

Special stain that identifies the presence of a structure

dormant form of bacterium that allows survival in poor environment

Front 23

Schaeffer Fulton Endospore Stain Procedure

Back 23

1.Prepare heat-fixed smear on clean slide.

2.Apply malachite green while steaming, 5 min. DO NOT let it dry out.

3.Cool slide for 2 min. Gently rinse w/distilled water.

4.Counterstain w/ safranin 1 min; rinse w/ water

5.Gently blot w/ bibulous paper

6.Observe under oil immersion

Front 24

Differential stain type

Back 24

Acid Fast Stain

Front 25

Mycobacterium and Nocardia

Back 25

are AF (+)

because cellwall contains waxy lipid called mycolic acid

Front 26

Mycolic Acid:

Back 26

-givescells higher affinity for carbolfuschin stain

-makescells resistant to decolorization by acid alcohol solutions

Front 27

Ziehl Neelsen

Back 27

1.Prepare heat-fixed smear on clean slide.

•Use separate aseptic transfers to add Mycobaterium laticola (mix into drop first and very thoroughly) andStaphylococcus aureus to the slide.

2.Applycabolfuchsinwhile steaming, 5 min.DO NOT let it dry out (add stain if drying out).

3.Cool30 sec and rinse w/ distilled water.

4.Decolorizew/ acid alcohol until run-off is clear (15-20sec, CAUTIONACID!)

5.Counterstainw/ methyleneblue (1 min)

6.Rinsew/ distilled water

7.Gentlyblot w/ bibulous paper

8.Observeunderoil immersion