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16 Cards in this Set
- Front
- Back
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What stain is the basic stain for Microbiology?
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Gram Stain
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What makes up the peptoglycan bridges?
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n-acetylmoramic (NAM)
N-acetylglucosomine (NAG) |
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What holds NAM and NAG together?
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B-1,4 Linkages
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Gram __ has a thin peptoglycan layer (lipopolysaccharide & protien)
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Negative
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Gram Staining:
Primary Stain? Mordant? Secondary Stain? |
Crystal Violet
Gram Iodine Safarin |
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In gram staining, what bacteria is cocci and which is baccillis?
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Cocci is Staphlycoccus Epidermidis
Baccillis is Serratia |
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____ is an acidic stain, therefore if you mistake it for Safarin it will only stain the background
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Congo Red
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What are capsules composed of?
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Polysaccharies or Polypeptides
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Which stain do you NOT heat fix?
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Capsule Staining
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For Capsule staining, if there is a clear capsule it will be (p/n)?
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Positive
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An acid fast stain is used to detect?
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Mycobacterium (TB and Leporasy)
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___ Stain - uses heating method to force stain into cell wall
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Acid Fast Stain
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In an Acid Fast Stain, Mycobacterium will always be (p/n) and ___ in color, and usually bacillis in shape.
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Positive - Pink
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Lipids are Gram ___
Polypeptoglycan is Gram ____ |
Negative
Positive |
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___ stain is dependent upon the presence of the keratin protien in wall.
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Endospore
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What stains are used during the Endospore Stain?
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Malachite Green
Saffarin |
