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18 Cards in this Set

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Phenol Red Glucose Broth w/Durham tube

Phenol Red Glucose(Acid/Gas)
(Test Tube #1)
Results:
Positive - Yellow/Bubble
Negative - Red/NO Bubble
Mixed Acid Fermentation
MR-VP Broth
MR Test

(Test Tube #4)
ADD 4 drops of Methyl Red

Results:
Positive - Red
Negative - Yellow
2, 3-Butanediol Fermentation
MR-VP Broth
VP Test

(Test Tube #5)
ADD 4 drops of Barritt’s reagent
VORTEX for 10-15 seconds
Then let the tube sit for about 30 minutes

Results:
Positive - Red
Negative - Yellow
Catalase Production
TSA Slant
Catalase Test

(Test Tube #6)
ADD 2 drops of hydrogen peroxide to the inoculated TSA slant.

Results:
NO Bubbles - Negative
Bubbles - Positive
Urea Hydrolysis (Broth)
Urease Test

(Test Tube #7)
Results:
Positive - Bright pink
Negative - Yellow
Tryptophan Hydrolysis (Broth)
Indole Test

(Test Tube #8)
ADD 12 drops of Kovac’s reagent.

Results:
Positive - Red Band
Negative - Yellow Band
Citrate Utilization
Simmons Citrate Agar Slant

Citrate Test
(Test Tube #9)
Results:
Positive - Prussian Blue
Negative - Green
Nitrate Production
Nitrate Broth

Nitrate Test
(Test Tube #10)
ADD 3 drops of reagent A
THEN add 3 drops of reagent B

Results:
Positive - DEEP red/orange
Negative - Yellow
Motility (SIM) Deep
(Test Tube #11)
Results:
Non-motile - grow ONLY along the line of inoculation(NEG)

Motile - organisms SPREAD OUT from the line of inoculation and may even grow throughout the medium (POSITIVE)
Triple Sugar Iron (TSI Slant)
Sugar Fermentation (SLANT)

(Test Tube #12)
Results:
Positive - Yellow
Negative - Red
Triple Sugar Iron (TSI Slant)
H2S Production(Hydrogen Sulfide)
(Test Tube #12)
Results:
Positive - BLACK
Negative - Red
Starch Hydrolysis
Starch Agar Plate
Pour enough Grams’ iodine over the surface of the plate to cover surface.

Results:
Positive - Clear Zone along streak
Negative - Dark/Black
Triple Sugar Iron (TSI Slant)
Sugar Fermentation(BUTT)
(Test Tube #12)
Results:
Positive - Yellow
Negative - Red
Triple Sugar Iron (TSI Slant)
CO2 Production (GAS)
(Test Tube #12)
Results:
Positive - Bubble
Negative - NO Bubble
Phenol Red Lactose Broth
Phenol Red Lactose(Acid)

(Test Tube #2)
Results:
Positive - Yellow
Negative - Red
Casein Hydrolysis
Skim Milk Agar
Results:
Positive - Clear Zone along streak
Negative - No clear area
Phenol Red Mannitol Broth
Phenol Red Mannitol(Acid)

(Test Tube #3)
Results:
Positive - Positive
Negative - Red
Gram Stain
1. Obtain a clean slide
2. Apply 2 loopfuls of WATER to slide
3. Aseptically remove a colony from your TSA plate culture and PLACE in water
4. AIR DRY
5. HEAT FIX
6. Perform Gram Stain (C - 1 MIN, I - 1 MIN, E - 3-5 SEC, S - 1 MIN)
7. Gram REACTION:
Positive - Purple
Negative - Pink
8. Cell Shape/Cell Arrangement