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14 Cards in this Set

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  • Back
Simmon's Cytrate Test
Agar slant
tube filled with (HOT)liquid agar then allowed to cool
Bottom is called butt
(for anerobic growth)
Contol is moss green if reaction turns blue
Light translucent straw colored
Neither differtial or selective
grows bacteria white to grey
Detect pigment
Red- Serratia
What is the greenish coloration due to the partial breakdown of blood in the plain blood plate?
Bilverdin an intermediate breakdown product of blood
(gives a greenish bruise color)
Plain blood plate
Triple Soy Agar Plate with 5% sheep blood
IS Differential NOT selective
If a bug really loves the blood it will leave a transparent ring around the colony= Beta Hemolysis
If a bug is only so so it will leave a greenish opaque ring around the colony= alpha hemolysis
gamma hemolysis =NO hemolysis
glucose/dextrose,lactose, and sucrose all in one it also contains iron and therefore tests for H2S
agar not broth will detect gas : froth on the slant, cracks in the agar and or space under the butt(where gas has accumulated
Some characteristics of bacterial colonies...
They can be round, smooth, wrinkled edges, shiny, dull or have a variety of colors
Litmus Milk
Neutral control color= opaque lavender
if bug eats it or breaks down it turns pink the acid in return creates renin in milk and it goes to the bottom of the tube as "curd"
someitmes a brownish ring at top which is peptinization (just a further breakdown)
What does selective media do?
It inhibits certain microbes from growing in or on a particular medium
Example of bug that sometimes causes peptonization?
Psuedomonas sp.
What is the TSIA a backup for?
the purple broth and sulphur parts of the sim test
How does selective media kill bugs?
with dyes, salts, or antibiotics
Serial Culture
1. Heat loop
2.flame rim
3.Dip loop in speciman
Dip loop in clean media
5. Heat loop sterilize

Loop, Lip,Lip, Loop
When do colonies form a culture?
about 2 days (48 hrs)
How long does the lag phase last?
Average of 2 hrs, but ranges from 1-48 hrs