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10 Cards in this Set
- Front
- Back
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facultative anaerobe
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can grow with or without oxygen. turbidity seen throughout agar
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aerobe
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growth should only be found on the surface
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anaerobe
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growth will not be near the top. gas produced by bacteria will cause breaks in the agar
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oxidation-reduction potential
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ratio of oxygen to hydrogen
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gram stain
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1. crystal violet
2. grams iodine 3. alcohol 4. safrannin |
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negative stain
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stained background rather than stained cells
stain air dries, so heat fixing is not needed not all bacterias absorb gram stain |
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acid fast stain
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primary stain, decolorizing agent, counterstain
acid fast bacteria will be red and not acid fast bacteria will be blue |
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spore stain
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endospore will take on the primary stain, water rinses the rest of the dye stain away, then counterstain the vegatative cell
endospore= green vegatative cell=red/pink |
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capsule stain
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primary stain and counter stain
pink bacterium surrounded by a light blue halo |
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aspetic technique
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open as briefly as possible
keep close to horizontal sterilize all tools before and after use or throw them away |
